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  1. Link

    native-human-collagen-iii-protein-ab7535.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Native Human Collagen III protein (ab7535)

  • Datasheet
  • SDS
Reviews (3)Q&A (3)References (3)

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Western blot - Native Human Collagen III protein (ab7535)

    Key features and details

    • Expression system: Native
    • Suitable for: IP, ELISA

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    Description

    • Product name

      Native Human Collagen III protein
      See all Collagen III proteins and peptides
    • Expression system

      Native
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Native
      • Species

        Human

    Associated products

    • Positive Controls

      • Recombinant Mouse Collagen III protein (His tag) (ab196093)

    Specifications

    Our Abpromise guarantee covers the use of ab7535 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Immunoprecipitation

      ELISA

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituent: 3% Acetic acid

    General Info

    • Alternative names

      • Alpha 1 type III collagen
      • Alpha1 (III) collagen
      • CO3A1_HUMAN
      • COL 3A1
      • COL3A1
      • Collagen alpha 1(III) chain
      • Collagen alpha-1(III) chain
      • Collagen III alpha 1 chain precursor
      • Collagen III alpha 1 polypeptide
      • Collagen type III alpha
      • Collagen type III alpha 1
      • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant)
      • Collagen type III alpha 1 chain
      • Collagen, fetal
      • EDS4A
      • Ehlers Danlos syndrome type IV, autosomal dominant
      • Fetal collagen
      • Type III collagen
      see all
    • Function

      Collagen type III occurs in most soft connective tissues along with type I collagen.
    • Involvement in disease

      Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
      Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
      Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
    • Sequence similarities

      Belongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 VWFC domain.
    • Post-translational
      modifications

      Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P02461 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Western blot - Native Human Collagen III protein (ab7535)
      Western blot - Native Human Collagen III protein (ab7535)Image courtesy of Michaela Leyh by Abreview.
      All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution

      Lane 1 : plus ab7535 reduced and denatured
      Lane 2 : plus ab7535 native

      Secondary
      All lanes : Goat anti-mouse-HRP at 1/2000 dilution


      The protein can be used reduced + denaturated (band size ~130 kDa) or native (two bands ~ 130kDa and ~300kDa) The native protein gives a brighter signal.

      PH of the protein lysate is low - a loading dye with bromphenolblue changes its colour. This does not effect the western blot.

      See Abreview

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab7535? Please let us know so that we can cite the reference in this datasheet.

    ab7535 has been referenced in 3 publications.

    • Di Francesco S  et al. Red grape (Vitis vinifera L.) flavonoids down-regulate collagen type III expression after UV-A in primary human dermal blood endothelial cells. Exp Dermatol 27:973-980 (2018). PubMed: 29742305
    • Wang Z  et al. Extracellular matrix promotes proliferation, migration and adhesion of airway smooth muscle cells in a rat model of chronic obstructive pulmonary disease via upregulation of the PI3K/AKT signaling pathway. Mol Med Rep 18:3143-3152 (2018). PubMed: 30066869
    • Kehlet SN  et al. A fragment of SPARC reflecting increased collagen affinity shows pathological relevance in lung cancer - implications of a new collagen chaperone function of SPARC. Cancer Biol Ther 19:904-912 (2018). PubMed: 30067436

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Collagen III sandwich ELISA

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Capture antibody Anti collagen III antibody ab6310

    Natural human protein Natural human collagen III protein ab7535 https://www.abcam.com/natural-human-collagen-iii-protein-ab7535.html

    Detection antibody Anti collagen III antibody biotin ab6580
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 07 2017

    Digestion og human collagen type III with MMP-13

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    SDS-PAGE
    Loading amount: 250 ng collagen
    Gel: Reduced denaturing (7% Tris acetate gel (Novex))
    Lane 1 standards
    Lane 2 blank
    Lane 3 MMP-13
    Lane 4 ab7533
    Lane 5 ab7533 + MMP-13 (62 ng)
    Lane 6 ab7533 + MMP-13 (31 ng)
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 18 2015

    Other (Western Blot) Abreview for Collagen III protein

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Other - Do not use

    Review text: The protein can be used reduced + denaturated (band size ~130 kDa)
    or native (two bands ~ 130kDa and ~300kDa)
    The native protein gives a brighter signal.

    PH of the proteinlysate is low - a loading dye with bromphenolblue changes its colour. This does not effect the western blot.
    Sample: Human Purified protein

    Primary antibody (in addition to 'Collagen III protein')
    Primary antibody: Abcam primary antibody: Anti-Collagen III antibody [FH-7A] (ab6310)
    Dilution: 1/500

    Secondary antibody
    Name: Non-Abcam antibody was used: goat anti mouse IgG-HRP, Santa Cruz-2005, lot c100
    Host species: Goat
    Clonality: Polyclonal
    Conjugation: Horse Radish Peroxidase
    Dilution: 1/2000
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Michaela Leyh

    Verified customer

    Submitted Feb 09 2011

    Question

    A migration assay with the latest vial of collagen III gave unexpected results, compared to a previous lot. Coomassie stain of gel revealed multiple small bands and weaker intensity, suggesting lower concentration and/or more degradation compared to the previous lot.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 10 2012

    Answer

    Thank you for contacting us.

    I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Abcam Scientific Support

    Answered on Jul 10 2012

    Question

    What is the Peptide region?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 03 2011

    Answer

    I realized that I may not have written down the ID number of the product you were interested in correctly. Could you please send me the correct abID number and I will be able to complete you request.

    Read More

    Abcam Scientific Support

    Answered on Nov 03 2011

    Question

    what is the cross reactivity of ab7533 and ab7535 with collagen type I?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 15 2005

    Answer

    These products have been prepared from human placenta and are chromatographically and immunologically pure. They are free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. Reaction with our anti-Collagen antibodies I, II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA) apart from the expect cross reaction with the collagen type i.e ab7535 cross reacts with the anti-collagen III antibody, and ab7533 cross reacts with the anti-collagen I antibody.

    Read More

    Abcam Scientific Support

    Answered on Jul 15 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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