Overview

Description

  • Nature

    Native
  • Source

    Native
  • Amino Acid Sequence
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab62386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Constituent: 50% Glycerol

General Info

  • Alternative names

    • Coagulation factor VII
    • coagulation factor VII (serum prothrombin conversion accelerator)
    • Eptacog alfa
    • F7
    • FA7_HUMAN
    • Factor VII
    • Factor VII heavy chain
    • Factor VII light chain
    • FVII coagulation protein
    • OTTHUMP00000018733
    • OTTHUMP00000018734
    • Proconvertin
    • Serum prothrombin conversion accelerator
    • SPCA
    see all
  • Function

    Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. FA7D is a rare hereditary hemorrhagic disease. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Numerous subjects are completely asymptomatic despite a very low F7 level.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab62386 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

The concentration of our factor VII is obtained by UV, using an extinction coefficient: E 1% 1cm , 280 nm = 13.9.

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Answer

Thanks for your reply and for your patience. I apologize for the extended delay in getting more information about this protein. My contact at the lab has said that the SDS-PAGE profile of reduced Factor VII and VIIa are distinct. Reduced and non reduced samples are run for each lot (see attached image). Reduced Factor VIIa runs as two bands at 30kDa and 20 kDa, so if there were Factor VIIa there would be two bands in the reduced (R) sample. We do not have further information about the cross-reactivity of the purifying antibody. Please let me know if you have any further questions and I will be happy to help.

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Question
Answer

Thank you for your call yesterday and for your patience while I have been in touch with the lab. Based on SDS-PAGE, the Factor VII protein ab62386 does not contain detectable levels of Factor VIIa. I hope this information will be useful to you, but please let me know if you have any further questions and I'll be happy to help.

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