Description

  • Product name

    Native Human Factor XIIIa protein
  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab62412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Enzymatic FXIIIa is composed of A2'B2. Ab62412 is the active, enzymatic tetrameric form (A2'B2) in absence of calcium ions. In contrast to the inactive tetrameric FXIII (A2B2), ab62412 has been activated by thrombin.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituents: 0.0146% EDTA, 50% Glycerol

General Info

  • Alternative names

    • bA525O21.1 (coagulation factor XIII, A1 polypeptide)
    • Coagulation factor XIII A chain
    • Coagulation factor XIII A1 polypeptide
    • Coagulation factor XIII A1 subunit
    • Coagulation factor XIII, A polypeptide
    • Coagulation factor XIIIa
    • F13A
    • F13A_HUMAN
    • F13a1
    • Factor XIIIA
    • Fibrin stabilizing factor, A subunit
    • Fibrinoligase
    • FSF, A subunit
    • Protein glutamine gamma glutamyltransferase A chain
    • Protein-glutamine gamma-glutamyltransferase A chain
    • TGase
    • Transglutaminase A chain
    • Transglutaminase, plasma
    • Transglutaminase. plasma
    see all
  • Function

    Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
  • Involvement in disease

    Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
  • Sequence similarities

    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Post-translational
    modifications

    The activation peptide is released by thrombin.
  • Cellular localization

    Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
  • Information by UniProt

Images

  • Lane 1 : Non-reduced Factor XIIIa protein (ab62412) at 1 µg
    Lane 2 : Molecular weight standard
    Lane 3 : Reduced Factor XIIIa protein (ab62412) at 1 µg Notes: In the non-reduced sample the A chain shifts down toward the B chain following activation.

References

ab62412 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Vielen Dank für Ihre Antwort. Leider haben wir aktuell kein aufgereinigtes A chain des Factors XIIIa oder Factors XIII in unserem Katalog. Ich habe auch das Labor die den ab62412 produziert angefragt, ob ein aufgereinigtes A chain erhältich sei, jedoch leider haben sie es auch nicht. Entschuldigen Sie bitte die Umstände. Ich hoffe, Sie finden ein Ihren Wünschen entsprechendes Protein. Ich kann auch die Webseite www.biocompare.com empfehlen, um nach Reagenzien zu suchen. Ich wünsche Ihnen viel Erfolg bei Ihrer Forschung. Bitte zögern Sie nicht, uns wieder zu kontaktieren, sollten Sie weitere Fragen haben.

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Answer

Thank you for your inquiry. Indeed, the protein ab62412, Factor XIIIa, is not recombinantly expressed, but the human purified protein. As expected therefore, the molecular weight is around 70kD. Please see the SDS-Page image in the attachment. I hope this information is useful. Please let me know if you have any additional question.

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Question
Answer

Thank you for contacting us. Activity of the proteins was assayed with a commercially available kit, as described below. (We do not offer the kit in our catalogue). The protein has not, to our knowledge, been used in any kind of functional assay of mouse blood but it may be possible to adapt the principle of the kit assay described below to your samples. Specific Activity: Assay is performed with specifications supplied with the Pefakit FXIII Incorporation Assay (Pentapharm LTD., Cat. # 501-00). Plasma based photometric determination of Factor XIII activity (See protocol included in the kit). Principle of the method - FXIII substrate fibrinogen (fbgn) is coated on the surface of a microtiter plate. Factor XIII in the sample is activated by thrombin and calcium ions (step omitted in the case of FXIIIa). In the incorporation step FXIIIa in the sample incorporates the substrate 5-biotinamidopentylamin (BAPA) into fbgn coated on the microtiter plate in the presence of calcium. The amount of incorporated BAPA is proportional to the FXIII activity of the sample. In the next step a strepavidine-alkaline phosphatase conjugate is bound to the incorporated BAPA. Alkaline phosphatase converts the synthetic substrate p-nitro phenyl phosphate into phosphate and p-nitrophenol which can be measured at 405 nm. Calculation of specific activity (Units/mg) - Results from the assay are reported as a percent (%) of NPP (based on controls provided by the assay kit). One unit is equal to the Factor XIII activity in 1 ml normal plasma (1 Unit = 100%). As an example 74.3% is equivalent to 0.74 U/ml. Divide U/ml by 101 to account for dilutions made in the standard curve. As in the example 0.74 U/ml / 101 = 0.0074 U/ml. Multiply this value by the dilution factor of the unknown sample (ex. 1:80,000) and divide by sample concentration (mg/ml). The result should be reported in Units/mg. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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