Description

  • Product name

    Native Human HDL protein
    See all HDL proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    >95% of total lipoprotein content by electrophoresis.
    Single arc by IEP against antisera to whole human serum.
    Essentially free from other plasma lipoproteins as determined by electrophoresis using Fat Red 7B staining for lipids and Coomassie Blue for proteins.
    Product is 0.2µm filtered.
  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab77897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    All human source materials have tested non-reactive for anti-HBc, anti-HCV and HBsAg and negative for anti-HIV1 and HIV2 by FDA required tests. No test guarantees a product to be non-infectious.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

    pH: 7.40
    Constituents: 0.01% EDTA, 0.87% Sodium chloride

General Info

  • Alternative names

    • High Density Lipoprotein
  • Relevance

    High-density lipoprotein (HDL) is one of the five major groups of lipoproteins, which, in order of sizes, largest to smallest, are chylomicrons, VLDL, IDL, LDL, and HDL, which enable lipids like cholesterol and triglycerides to be transported within the water-based bloodstream. In healthy individuals, about thirty percent of blood cholesterol is carried by HDL.

    Blood tests typically report HDL-C level, i.e. the amount of cholesterol contained in HDL particles. It is often contrasted with low-density or LDL cholesterol or LDL-C. HDL particles are able to remove cholesterol from within artery atheroma and transport it back to the liver for excretion or re-utilization, which is the main reason why the cholesterol carried within HDL particles (HDL-C) is sometimes called "good cholesterol" (despite the fact that it is exactly the same as the cholesterol in LDL particles).

References

ab77897 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

The molecular weight for ab77897 is 175,000 to 360,000. The major protein component is Apo AI.

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Question

Finally a reply ;) thank you for the answer on my inquiry.



Regarding the ApoA-I or HDl, yes, since we isolate our HDL and ApoA-I from human plasma and check for quality, we probably have higher quality material than one can buy (we compared some years ago with other companies ApoA-I one can buy, but not with abcam’s).



how do you get the HDL2 fraction in ab77881 by centrifuging 1.063-1.210? by this densities you would isolate total HDL, not only HDL2.



ab77897 - purity > 95% by electrophoresis, but then how can it be, that its “Essentially free for other plasma lipoproteins”? by 95% you allow 5% to be some other lipoproteins or plasma proteins?





Regarding indirect immunofluorescence of HDL inside cells:

ab52945 we already got from abcam in 2011 and unfortunately in indirect immunofluorescence it is not seeing the HDL particles taken up by endothelial cells.

The other 4 antibodies you listed are probably also only against ApoA-I but not HDL and even worse (since the ab52945 is rab monoclonal), but we cannot buy them all and test just for testing. All your references and pictures on the fact sheet pages online are in (liver/liver-originating) cells producing ApoA-I which is prepared for secretion by the cells (meaning not yet lipidated) and not ApoA-I or HDL from circulation (or isolated plasma) that is taken up by the cells. Our system is: cells taking up HDL and from all my experiments HDL or reconstituted HDL is not seen by yet all anti ApoA-I or anti HDL antibodies in immunofluorescence.



If it is possible to get free samples of the antibodies classified for indirect immunofluorescence to test for HDL taken up in cells for free, I’m willing to try and report to you what came out. For now, the only way to see HDL trafficking inside cells is by directly labeling it.



Best regards,

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Answer

Thank you for your answer.

I am copying the answer form our lab directly to not make a mistake:

1.) For ab77881, we did not mean to say that total HDL would not also be isolated, just that we would have approximately equal amounts of HDL2 and HDL3.

2.) Per the lab for ab77897, minimum purity is > 95%, so therefore, less than 5% will be other plasma proteins (which is considered essentially free by the lab).

I am sorry to confirm that we cannot offer free samples. This is logistically not possible since we have over 100000 products in our catalog.

We cannot make any predictions which antibody would also recognize lipidated HDL and even if tested positive would not be able to guarantee that for all batches of an antibody.

I am sorry to confirm that this is not eligible for the testing program and apologize for then inconvenience.

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Answer

Thank you for your inqury.


We have antibodies that are tested and guaranteed for IHC-Fr and ICC which are the main applictaions for indirect immunofluorescence:

ab52945

https://www.abcam.com/index.html?datasheet=52945 (or use the following: https://www.abcam.com/index.html?datasheet=52945).

ab33470

https://www.abcam.com/index.html?datasheet=33470 (or use the following: https://www.abcam.com/index.html?datasheet=33470).

ab20918

https://www.abcam.com/index.html?datasheet=20918 (or use the following: https://www.abcam.com/index.html?datasheet=20918).

ab58924

https://www.abcam.com/index.html?datasheet=58924 (or use the following: https://www.abcam.com/index.html?datasheet=58924).

ab34788

https://www.abcam.com/index.html?datasheet=34788 (or use the following: https://www.abcam.com/index.html?datasheet=34788).

Please follow this link if you are interested in purchasing an alternative secondary antibody.

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

These are the specifications for ab77897, ab80476 and ab77881 for HDL:

ab77881

HDL2 Subfraction
Not tested for purity
Ultracentrifugation at density 1.063-1.210
100 mM Sodium Chloride, 10 mM Tris, 1 mM EDTA, pH 7.4
1 mM Sodium Azide
Devoid of VLDL, IDL and LDL


ab77897

Mainly contains apoliprotein AI
purity > 95% by electrophoresis
Sequential flotation ultracentrifugation technique using Potassium Bromide for density adjustments
150 mM Sodium Chloride, 0.01% EDTA, pH 7.4
Essentially free for other plasma lipoproteins


ab80476

˜equivalent amounts of HDL2 and HDL3
Not tested for purity
Ultracentrifugation at density 1.063-1.210
100 mM Sodium Chloride, 10 mM Tris, 1 mM EDTA, pH 7.4
1 mM Sodium Azide
Devoid of VLDL, IDL and LDL


Difference between ab50239, ab66674 and ab90760 for ApoA-I

ab66674 is only a short pepetide and is only used for blocking purposes and therefore not suitable for your application.

ab50239 and ab90760 are both full length recombinant proteins. Both do not contain preservatives and have a purity > 95%. For ab50239 the endotoxin level has been tested and is less than 0.1 ng per µg (1EU/µg).

I hope this information is helpful and wish you good lucvk with your research.

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Answer

Thank you for your enquiry. I would be pleased to help answer your questions: 1. Is Human HDL. Yes, I can confirm this is HDL from human as stated in the product title and on the online datasheet. 2. Is this the highest grade of pure HDL you sell? Reviewing the details we have, this is the purist of the HDL proteins we sell. 3. What is the density of this lipoprotein? The density is 1.063-1.210 g/mL. 4. Apoliprotein A I & Apo II would be bound to this protein, is this correct? The major protein component is Apo AI. 5. Do you have a product for Mouse HDL? I am sorry to confirm that we regrettably do not have a mouse HDL protien in the Abcam catalogue. The purifity information we have for this protein is as follows: >95% of total lipoprotein content by electrophoresis. Single arc by IEP against antisera to whole human serum. Essentially free from other plasma lipoproteins as determined by electrophoresis using Fat Red 7B staining for lipids and Coomassie Blue for proteins. Product is 0.2µm filtered. I can recommend to review the online datasheet for further information: Click here (or use the following: https://www.abcam.com/index.html?datasheet=77897). I hope this will be helpful to you. Should you have any further question, please do not hesitate to contact us.

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Answer

Thank you for your inquiry. ab77881, ab77897, ab80476 have more differences than the concentration and purification you have noticed. ab80476 is specific for the HDL2 subfraction and is devoid of HDL3 while the other two are specific for HDL. ab77897 is not from human normolipidemic plasma. Each lot is from a single donor. Examples of cholesterol and triglyceride values from previous batches of this product: Cholesterol = 5.12 mg/mL Trig = 1.68 mg/mL Cholesterol = 3.92 mg/mL Trig = 1.32 mg/mL Cholesterol = 5.71 mg/mL Trig = 2.16 mg/mL Cholesterol = 6.06 mg/mL Trig = 2.25 mg/mL The purification using ultracentrifugation is the same for all 3. ab77897 is also filtered with a 0.2 um filter. Concentrations will change from batch to batch for all 3 products. ab77897 buffer does not contain Tris nor does it have a preservative while the other 2 contain Tris and have a preservative.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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