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Hello, I am interested in purchasing some Human HDL protein. I see that you offer 3 different items: ab77881, ab77897 & ab80476. I would like to know how these products differ & it is not really apparent from the data sheets (other than the fact that ab77897 has no sodium azide in the storage buffer). Are they all purified similarly? ab77881 & ab80476 look identical on the datasheet, with the exception of the protein concentration, so can you please clarify what the differences are? Also ab77897 is listed as "full length native mature human HDL purified from human plasma" where as the other 2 products are listed as just "full length mature human HDL" - so my question for you is what do you mean by "native" for ab77897 & is that truly different than the other 2 products? Lastly ab77881 & ab80476 are listed as being purified from normolipidemic plasma, but this is not specified for ab77897 - so can you tell me if ab77897 is also purified from normolipidemic plasma or not? Thank you for your assistance,
Asked on Aug 06 2010
Thank you for your inquiry. ab77881, ab77897, ab80476 have more differences than the concentration and purification you have noticed. ab80476 is specific for the HDL2 subfraction and is devoid of HDL3 while the other two are specific for HDL. ab77897 is not from human normolipidemic plasma. Each lot is from a single donor. Examples of cholesterol and triglyceride values from previous batches of this product: Cholesterol = 5.12 mg/mL Trig = 1.68 mg/mL Cholesterol = 3.92 mg/mL Trig = 1.32 mg/mL Cholesterol = 5.71 mg/mL Trig = 2.16 mg/mL Cholesterol = 6.06 mg/mL Trig = 2.25 mg/mL The purification using ultracentrifugation is the same for all 3. ab77897 is also filtered with a 0.2 um filter. Concentrations will change from batch to batch for all 3 products. ab77897 buffer does not contain Tris nor does it have a preservative while the other 2 contain Tris and have a preservative.
Answered on Aug 06 2010