Key features and details
- Expression system: Native
- Suitable for: SDS-PAGE
Product nameNative Human Hemoglobin A1c protein
Purity96 % .
ab98306 was sterile filtered through a 0.2µm membrane. Purity >96% as determined by FPLC using a Mono S Haemoglobin A1c column (dispensing solution).
Protein lengthFull length protein
Predicted molecular weight64 kDa
Amino acids2 to 142
Our Abpromise guarantee covers the use of ab98306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab98306 is the full tetrameric form of Hemoglobin, although on SDS-PAGE it will show as a mixture of the tetrameric/monomeric forms as the SDS denatures the protein to some extent.
Starting material donor tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
FunctionInvolved in oxygen transport from the lung to the various peripheral tissues.
LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure.
Spinorphin: functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.
Tissue specificityRed blood cells.
Involvement in diseaseHeinz body anemias
Sickle cell anemia
Beta-thalassemia, dominant, inclusion body type
Sequence similaritiesBelongs to the globin family.
modificationsGlucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus.
S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2).
Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure. PubMed:16916647 reports the identification of HBB acetylated on Lys-145 in the cytosolic fraction of HeLa cells. This may have resulted from contamination of the sample.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab98306 has not yet been referenced specifically in any publications.