Description

  • Product name

    Native Human Hemoglobin A1c protein
  • Purity

    96 % .
    ab98306 was sterile filtered through a 0.2µm membrane. Purity >96% as determined by FPLC using a Mono S Haemoglobin A1c column (dispensing solution).
  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human
    • Predicted molecular weight

      64 kDa
    • Amino acids

      2 to 142

Specifications

Our Abpromise guarantee covers the use of ab98306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    ab98306 is the full tetrameric form of Hemoglobin, although on SDS-PAGE it will show as a mixture of the tetrameric/monomeric forms as the SDS denatures the protein to some extent.

    Starting material donor tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.

    Proprietary buffer formulation, pH 8.0.

General Info

  • Alternative names

    • Beta-globin
    • HBB
    • HBB_HUMAN
    • Hemoglobin alpha chain
    • Hemoglobin beta chain
    • heterotetrameric hemoglobin
    • Spinorphin
    • subunit alpha
    see all
  • Function

    Involved in oxygen transport from the lung to the various peripheral tissues.
    LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure.
    Spinorphin: functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.
  • Tissue specificity

    Red blood cells.
  • Involvement in disease

    Heinz body anemias
    Beta-thalassemia
    Sickle cell anemia
    Beta-thalassemia, dominant, inclusion body type
  • Sequence similarities

    Belongs to the globin family.
  • Post-translational
    modifications

    Glucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus.
    S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2).
    Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure. PubMed:16916647 reports the identification of HBB acetylated on Lys-145 in the cytosolic fraction of HeLa cells. This may have resulted from contamination of the sample.
  • Information by UniProt

References

ab98306 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

The haemoglobin A1c ab98306 is a native protein and prepared from packed red blood cells; the protein should be intact, so it is glycated full length.

 The tetramer molecular weight is 64KDa consisting of 4 subunits of 16KDa

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