Native Human IgA protein (ab91025)

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Overview

  • Product name
    Native Human IgA protein
  • Protein length
    Full length protein

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Human
    • Additional sequence information
      Prepared from plasma.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab91025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes
    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.32 Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.05% Sodium Azide
    Constituents: 100mM Sodium chloride, 100mM Tris HCl, pH 8.0

General Info

  • Alternative names
    • Hepatocellular carcinoma-associated protein TB6
    • Ig alpha 1 chain C region
    • Ig alpha 2 chain C region
    • IGHA
    • IGHA1
    • IGHA2
    • Immunoglobulin heavy constant alpha 1
    • Immunoglobulin heavy constant alpha 2
    • Immunoglobulin heavy constant alpha 2 A2m marker
    • PIgR
    • Poly-Ig receptor
    • polymeric immunoglobulin receptor
    • polymeric immunoglobulin receptor Secretory component
    see all
  • Relevance
    Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
  • Cellular localization
    Secreted

Images

  • SDS-PAGE - IgA protein (Human) (ab91025)
    SDS-PAGE - IgA protein (Human) (ab91025)
    SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    Lane 1: 5 µg IgA (reduced/heated)
    Lane 2: 10 µg IgA (reduced/heated)
    Lane 3: 20 µg IgA (reduced/heated)
    Lane 4: Molecular weight markers
    Lane 5: 5 µg IgA (non-reduced/no heat)
    Lane 6: 10 µg IgA (non-reduced/no heat)
    Lane 7: 20 µg IgA (non-reduced/no heat)

References

ab91025 has not yet been referenced specifically in any publications.

Publishing research using ab91025? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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5 reviews or Q&A

10
Votes: Highest First

How was this product (ab91025) purified? Is there any possibility of contaminating IgG in this product?


The purification of IgA involves an ammonium sulfate precipitation as well as a series of chromatographic steps to separate IgA from the other immunoglobulins.

Based on IEP results, there are no detectable IgG, IgM, IgE, or IgD contamina...

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Inquiry: Hi. I need a human IgA as a standard for a sandwich ELISA, wich is the difference betwen ab91025 and ab167824? Is it just the purity?

Thank you for contacting us.

Yes, both proteins are essentially the same with different purity.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Dear,

I am planning to use ab91025 in constructing a standard curve with known concentrations to interpolate the concentration of my samples. This will be performed by dot blot, so ab91025-standards together with my samples are blotted on a ...

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I think that data would be valuable to our customers so if you could share your results with us through an abreview that would be very useful. Your testing discount is as follows:

Discount code: xxxxxx


Expiry date: 2012-06-04 <...

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Phone call from customer wanting to perform dot blot with ab91025.

Thank you very much for your interest in ab91025.

To our knowledge, ab91025 has not been testedused to perform a dot blot. Therefore, I can offer a discount off a future purchase if you buy ab91025 now, test it indot blotand submit feedback ...

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Dear Sir/Madame, In general, what is the shelf life of antibodies from date received if aliquoted and stored at -20degrees? Kind regards,

Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage ins...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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