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  1. Link

    native-human-iga-protein-ab91025.pdf

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Immunology Immunoglobulins Heavy Chain IgA
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Native Human IgA protein (ab91025)

  • Datasheet
Submit a review Q&A (3)References (1)

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SDS-PAGE - Native Human IgA protein (ab91025)

    Key features and details

    • Expression system: Native
    • Purity: > 95% SDS-PAGE
    • Suitable for: SDS-PAGE

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    Description

    • Product name

      Native Human IgA protein
      See all IgA proteins and peptides
    • Purity

      > 95 % SDS-PAGE.

    • Expression system

      Native
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Native
      • Species

        Human
      • Predicted molecular weight

        160 kDa
      • Additional sequence information

        Prepared from plasma.

    Associated products

    • Related Products

      • Biotin Anti-IgA antibody [IgA5-3B] (ab53270)

    Specifications

    Our Abpromise guarantee covers the use of ab91025 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Additional notes

      Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.32 Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Preservative: 0.05% Sodium azide
      Constituents: 1.58% Tris HCl, 0.58% Sodium chloride

    General Info

    • Alternative names

      • Hepatocellular carcinoma-associated protein TB6
      • Ig alpha 1 chain C region
      • Ig alpha 2 chain C region
      • IGHA
      • IGHA1
      • IGHA2
      • Immunoglobulin heavy constant alpha 1
      • Immunoglobulin heavy constant alpha 2
      • Immunoglobulin heavy constant alpha 2 A2m marker
      • PIgR
      • Poly-Ig receptor
      • polymeric immunoglobulin receptor
      • polymeric immunoglobulin receptor Secretory component
      see all
    • Relevance

      Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
    • Cellular localization

      Secreted

    Images

    • SDS-PAGE - Native Human IgA protein (ab91025)
      SDS-PAGE - Native Human IgA protein (ab91025)
      SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
      Lane 1: 5 μg IgA (reduced/heated)
      Lane 2: 10 μg IgA (reduced/heated)
      Lane 3: 20 μg IgA (reduced/heated)
      Lane 4: Molecular weight markers
      Lane 5: 5 μg IgA (non-reduced/no heat)
      Lane 6: 10 μg IgA (non-reduced/no heat)
      Lane 7: 20 μg IgA (non-reduced/no heat)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab91025? Please let us know so that we can cite the reference in this datasheet.

    ab91025 has been referenced in 1 publication.

    • Biswas S  et al. IgA transcytosis and antigen recognition govern ovarian cancer immunity. Nature N/A:N/A (2021). PubMed: 33536615

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    How was this product (ab91025) purified? Is there any possibility of contaminating IgG in this product?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 20 2013

    Answer


    The purification of IgA involves an ammonium sulfate precipitation as well as a series of chromatographic steps to separate IgA from the other immunoglobulins.

    Based on IEP results, there are no detectable IgG, IgM, IgE, or IgD contaminants in our final product of IgA. Also, SDS-PAGE does not show a band in the region where heavy chain IgG would run.

    Read More

    Ariana Veiga

    Abcam Scientific Support

    Answered on Nov 20 2013

    Question


    Inquiry: Hi. I need a human IgA as a standard for a sandwich ELISA, wich is the difference betwen ab91025 and ab167824? Is it just the purity?

    Read More

    Abcam community

    Verified customer

    Asked on May 22 2013

    Answer

    Thank you for contacting us.

    Yes, both proteins are essentially the same with different purity.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on May 22 2013

    Question

    Dear Sir/Madame, In general, what is the shelf life of antibodies from date received if aliquoted and stored at -20degrees? Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 23 2011

    Answer

    Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies and other products like proteins, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least six month from the date of purchase when stored correctly. For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols). I hope this information helps. Please do not hesitate to contact us if you need anything further.

    Read More

    Abcam Scientific Support

    Answered on Dec 23 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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