Native Human IgA protein (ab91025)

Submit a review Q&A (5)

Overview

  • Product name

    Native Human IgA protein

  • Protein length

    Full length protein

Description

  • Nature

    Native

  • Source

    Native
  • Amino Acid Sequence

    • Species

      Human

    • Additional sequence information

      Prepared from plasma.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab91025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity

    > 95 % SDS-PAGE.

  • Form

    Liquid

  • Additional notes

    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.32 Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 0.05% Sodium azide
    Constituents: 1.58% Tris HCl, 0.58% Sodium chloride

General Info

  • Alternative names

    • Hepatocellular carcinoma-associated protein TB6
    • Ig alpha 1 chain C region
    • Ig alpha 2 chain C region
    • IGHA
    • IGHA1
    • IGHA2
    • Immunoglobulin heavy constant alpha 1
    • Immunoglobulin heavy constant alpha 2
    • Immunoglobulin heavy constant alpha 2 A2m marker
    • PIgR
    • Poly-Ig receptor
    • polymeric immunoglobulin receptor
    • polymeric immunoglobulin receptor Secretory component
    see all

  • Relevance

    Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.

  • Cellular localization

    Secreted

Images

  • SDS-PAGE - Native Human IgA protein (ab91025)
    SDS-PAGE - Native Human IgA protein (ab91025)
    SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    Lane 1: 5 μg IgA (reduced/heated)
    Lane 2: 10 μg IgA (reduced/heated)
    Lane 3: 20 μg IgA (reduced/heated)
    Lane 4: Molecular weight markers
    Lane 5: 5 μg IgA (non-reduced/no heat)
    Lane 6: 10 μg IgA (non-reduced/no heat)
    Lane 7: 20 μg IgA (non-reduced/no heat)

References

ab91025 has not yet been referenced specifically in any publications.

Publishing research using ab91025? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Question

How was this product (ab91025) purified? Is there any possibility of contaminating IgG in this product?

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Answer


The purification of IgA involves an ammonium sulfate precipitation as well as a series of chromatographic steps to separate IgA from the other immunoglobulins.

Based on IEP results, there are no detectable IgG, IgM, IgE, or IgD contaminants in our final product of IgA. Also, SDS-PAGE does not show a band in the region where heavy chain IgG would run.

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Question


Inquiry: Hi. I need a human IgA as a standard for a sandwich ELISA, wich is the difference betwen ab91025 and ab167824? Is it just the purity?

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Answer

Thank you for contacting us.

Yes, both proteins are essentially the same with different purity.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Dear,

I am planning to use ab91025 in constructing a standard curve with known concentrations to interpolate the concentration of my samples. This will be performed by dot blot, so ab91025-standards together with my samples are blotted on a nitrocellulose membrane, detected using an HRP-labeled secondary anti-IgA antibody followed by DAB staining.

The same experiment will be performed using human IgG protein standard + HRP-labeled secondary anti-IgG antibody, and human IgM protein standard + HRP-labeled secondary anti-IgM antibody (human IgG/IgM antibodies and anti-IgG/IgM secondary antibodies are already at my disposal).

For me it is very important that the unlabeled human Ig proteins serve as good standards, and that no cross-reaction occurs as my samples contain the three different isotypes and I am interested in quantifying them all three.

If the results of this application using ab91025 would be useful for you, I am willing to write an abreview and accept this offer.

Best regards,

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Answer

I think that data would be valuable to our customers so if you could share your results with us through an abreview that would be very useful. Your testing discount is as follows:

Discount code: xxxxxx


Expiry date: 2012-06-04

Once you have used theab91025 for the dot blot experiments, simply add an Abreview for this product and quote the discount code in the "“Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site:

https://www.abcam.com/Abreviews

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:

https://www.abcam.com/collaborationdiscount

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Question

Phone call from customer wanting to perform dot blot with ab91025.

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Answer

Thank you very much for your interest in ab91025.

To our knowledge, ab91025 has not been testedused to perform a dot blot. Therefore, I can offer a discount off a future purchase if you buy ab91025 now, test it indot blotand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab91025 in dot blot. I will then send a discount code. This code must be issued before purchasing ab91025 so please wait for my reply before ordering.

2. Purchase ab91025 either by phone, fax, or online (www.abcam.com).

3. Test it in dot blot.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit:

https://www.abcam.com/abreviews

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab91025 turns out to be unsuitable for dot blot, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at:

https://www.abcam.com/collaborationdiscount

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Question

Dear Sir/Madame, In general, what is the shelf life of antibodies from date received if aliquoted and stored at -20degrees? Kind regards,

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Answer

Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies and other products like proteins, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least six month from the date of purchase when stored correctly. For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols). I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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