Native Human IgA protein (ab91025)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
Description
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Product name
Native Human IgA protein
See all IgA proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Predicted molecular weight
160 kDa -
Additional sequence information
Prepared from plasma.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab91025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.32 Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 0.05% Sodium azide
Constituents: 1.58% Tris HCl, 0.58% Sodium chloride
General Info
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Alternative names
- Hepatocellular carcinoma-associated protein TB6
- Ig alpha 1 chain C region
- Ig alpha 2 chain C region
see all -
Relevance
Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen. -
Cellular localization
Secreted
Images
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SDS-PAGE - Native Human IgA protein (ab91025)SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
Lane 1: 5 μg IgA (reduced/heated)
Lane 2: 10 μg IgA (reduced/heated)
Lane 3: 20 μg IgA (reduced/heated)
Lane 4: Molecular weight markers
Lane 5: 5 μg IgA (non-reduced/no heat)
Lane 6: 10 μg IgA (non-reduced/no heat)
Lane 7: 20 μg IgA (non-reduced/no heat)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab91025 has been referenced in 1 publication.
- Biswas S et al. IgA transcytosis and antigen recognition govern ovarian cancer immunity. Nature N/A:N/A (2021). PubMed: 33536615
