Native Human IgA1 protein (ab91020)

Submit a review Q&A (2)References (1)
SDS-PAGE - Native Human IgA1 protein (ab91020)

    Key features and details

    • Expression system: Native
    • Purity: > 95% SDS-PAGE
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Native Human IgA1 protein

    • Biological activity

      No reaction by IEP to IgA2 antiserum.

    • Purity

      > 95 % SDS-PAGE.

    • Expression system

      Native

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Native

      • Species

        Human

    Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab91020 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        SDS-PAGE

      • Form

        Liquid

      • Additional notes

        Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.32. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.No reaction by IEP to IgA2 antiserum.
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped on Dry Ice. Store at -20°C.

        pH: 7.40
        Preservative: 0.05% Sodium azide
        Constituents: 0.0268% PBS, 0.87% Sodium chloride

      General Info

      • Alternative names

        • Ig alpha 1 chain C region
        • IGHA1
        • immunoglobulin heavy constant alpha 1

      • Relevance

        Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.

      • Cellular localization

        Secreted

      Images

      • SDS-PAGE - Native Human IgA1 protein (ab91020)
        SDS-PAGE - Native Human IgA1 protein (ab91020)
        SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
        1. 5 μg Human IgA1 (reduced /heated)
        2. 10 μg Human IgA1 (reduced/ heated)
        3. 20 μg Human IgA1 (reduced/ heated)
        4. Molecular weight markers
        5. 5 μg Human IgA1 (non-reduced/no heat)
        6. 10 μg Human IgA1 (non-reduced/no heat)
        7. 20 μg Human IgA1 (non-reduced/no heat)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (1)

      Publishing research using ab91020? Please let us know so that we can cite the reference in this datasheet.

      ab91020 has been referenced in 1 publication.

      • Sánchez-Salguero E  et al. Infectious episodes during pregnancy, at particular mucosal sites, increase specific IgA1 or IgA2 subtype levels in human colostrum. Matern Health Neonatol Perinatol 5:9 (2019). PubMed: 31205733

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      I am looking for IgA1 and IgA2 proteins to use as reference material in an ELISA. I was wondering how your two products 91020 and 91021 have been purified.

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      Answer

      For IgA1, isolated from human myeloma plasma, (ab91020) and IgA2, isolated from human myeloma plasma (ab91021), we perform an initial Ammonium sulfate precipitation. After, specific affinity columns are used based on the contaminants present. For example, the last time this product was made, we used Affigel Blue (BioRAD) and Gamma-bind for the removal of Albumin and IgG respectively.

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      Question

      Dear Sir/Madam, we are looking for the purified human myeloma IgA1 (Ale) protein, in polymeric form if possible. We woluld like to use in ELISA as a standard or control sample. I found the product human IgA1 protein in your catalog. Is this form mainly polimeric and could it be used in ELISA testsn as well? Is there anything know about the glycosylation of your purified protein? Do you have any other suitable protein? Thank you for the answer. Best regards,

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      Answer

      Thank you very much for your interest in ab91020. We unfortunately have not done any test to check whether this protein is polymeric or not. We also do not know if the protein is glycosylated. The molecular weight of monomeric IgA is 160 kDa. Theoretically the image represents a band at ˜150-160 kDa in non reduced/ no heat part of the gel which could represent the monomeric form of theprotein; we are however unable to confirm due to lack of tested data. If you are interested in testing this antibody then I can offer a discount off a future purchase if you buy ab91020 now, test it inIndirect ELISAand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free (PRIMARY ANTIBODY). If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab91020 in Indirect ELISA. I will then send a discount code. This code must be issued before purchasing ab91020 so please wait for my reply before ordering. 2. Purchase ab91020 either by phone, fax, or online (www.abcam.com). 3. Test it in Indirect ELISA. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab91020 turns out to be unsuitable for Indirect ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

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