Description

  • Product name

    Native Human IgA2 protein
    See all IgA2 proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab91021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.32. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. No reaction by IEP to IgA1 antiserum.

     

    No reaction by IEP to IgA1 antiserum.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.0268% PBS, 0.87% Sodium chloride

General Info

  • Alternative names

    • A2m marker
    • Ig alpha 2 chain C region
    • IgA constant heavy chain 2
    • IGHA 2
    • IGHA2
    • IGHA2 protein
    • Immunoglobulin A2
    • Immunoglobulin Am2
    • Immunoglobulin heavy constant alpha 2
    • immunoglobulin heavy constant alpha 2 (A2m marker)
    see all
  • Relevance

    Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
  • Cellular localization

    Secreted

Images

  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    1. 5 μg IgA2 (reduced /heated)
    2. 10 μg IgA2 (reduced/ heated)
    3. 20 μg IgA2 (reduced/ heated)

References

ab91021 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

For IgA1, isolated from human myeloma plasma, (ab91020) and IgA2, isolated from human myeloma plasma (ab91021), we perform an initial Ammonium sulfate precipitation. After, specific affinity columns are used based on the contaminants present. For example, the last time this product was made, we used Affigel Blue (BioRAD) and Gamma-bind for the removal of Albumin and IgG respectively.

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