Question (75033) | Native Human IgA2 protein (ab91021)

Go to datasheet (ab91021)

Question

I am looking for IgA1 and IgA2 proteins to use as reference material in an ELISA. I was wondering how your two products 91020 and 91021 have been purified.

Answer

For IgA1, isolated from human myeloma plasma, (ab91020) and IgA2, isolated from human myeloma plasma (ab91021), we perform an initial Ammonium sulfate precipitation. After, specific affinity columns are used based on the contaminants present. For example, the last time this product was made, we used Affigel Blue (BioRAD) and Gamma-bind for the removal of Albumin and IgG respectively.