Human IgE from Abcam (ab65866) was used as standard to prepare the calibration curve in PBS and in human serum matrix (attached figure). Two immunoassay configurations were assayed (competitive and sandwich format), obtaining better detection limit with the competitive format (0.2 ng/mL). For both formats the detection limits were better than those obtained with current commercial ELISA kits (12 ng/mL).
Moreover, the developed fluorescent competitive immunoassay offers other interesting advantages such as fewer reagents and simpler procedures.
The applicability of this new AuNCs immunoprobe was successfully demonstrated for the determination of IgE in human serum, using the competitive configuration.
This research has been published in Biosensors and Bioelectronics,
Authors: María Cruz Alonso, Laura Trapiella-Alfonso, José M Costa Fernández, Rosario Pereiro, Alfredo Sanz-Medel
Title: “Functionalized gold nanoclusters as fluorescent labels for immunoassays: Application to Human serum immunoglobulin E determination”
Submitted Sep 29 2015