Description

  • Product name

    Native Human IgG Fab fragment protein
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native

Associated products

Specifications

Our Abpromise guarantee covers the use of ab90352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm = 1.35.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.0536% PBS, 0.87% Sodium chloride

General Info

  • Relevance

    Human IgG consists of four subclasses (1-4) that can be recognised by antigen differences in their heavy chains. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens. The Fab fragment (fragment antigen binding) is the antigen region of the IgG molecule. It is composed of one constant and one variable domain of each of the heavy and the light chain.

References

ab90352 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

Unfortunately, the anti-human secondary antibody would bind both the protein and the primary antibody, resulting in a reduced signal, but not necessarily in a lower background.


The trick is to block the endogenous human antibodies before you incubate with your primary antibody, hence blocking all possibly unwanted binding sites for your secondary antibody without interferingwith the primary antibody.


For this reason, I would like to suggest ab98607 Rabbit F(ab')2 polyclonal Secondary Antibody to Human IgG - H&L, pre-adsorbed. Unfortunately, we do not have a Fab fragment antibody, which would be better as they are smaller and cover up more, but this one should do it still nicely:


https://www.abcam.com/index.html?datasheet=98607 (or use the following: https://www.abcam.com/index.html?datasheet=98607).



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.




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