Native Human IgG protein (ab98981)

Submit a review Q&A (7)References (1)
ELISA - Native Human IgG protein (ab98981)

    Key features and details

    • Expression system: Native
    • Suitable for: RID, SDS-PAGE

    Description

    • Product name

      Native Human IgG protein

    • Purity

      96 % .
      IgA and IgM levels are <1% of the total IgG content by RID. ab98981 shows a single band corresponding to gamma electrophoretic mobility by Cellulose Acetate Electrophoresis.

    • Expression system

      Native

    • Accession

      P01857

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Native

      Specifications

      Our Abpromise guarantee covers the use of ab98981 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        Radial Immunodiffusion

        SDS-PAGE

      • Form

        Lyophilized

      • Additional notes

        Starting material serum donor tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies.

        IgG from pooled normal human serum/plasma

      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C.

        Constituent: 0.158% Ammonium bicarbonate

      • Reconstitution
        Reconstitution in phosphate buffer, pH >7.0 containing 0.15M NaCl is recommended.

      General Info

      • Alternative names

        • FLJ39988
        • FLJ40036
        • FLJ40253
        • FLJ40587
        • FLJ40789
        • FLJ40834
        • G1m marker
        • G2m marker
        • G3m marker
        • G4m marker
        • HDC
        • Heavy chain disease protein
        • Human immunglobulin G
        • Ig gamma 1 chain C region
        • Ig gamma 2 chain C region
        • Ig gamma 3 chain C region
        • Ig gamma 4 chain C region
        • IgG heavy chain locus
        • IGHG 1
        • IGHG 2
        • IGHG 3
        • IGHG 4
        • IGHG1
        • IGHG2
        • IGHG3
        • IGHG4
        • Immunoglobulin Gm1
        • Immunoglobulin Gm3
        • Immunoglobulin Gm4
        • Immunoglobulin heavy constant gamma 1
        • immunoglobulin heavy constant gamma 1 (G1m marker)
        • Immunoglobulin heavy constant gamma 2
        • immunoglobulin heavy constant gamma 2 (G2m marker)
        • Immunoglobulin heavy constant gamma 3
        • immunoglobulin heavy constant gamma 3 (G3m marker)
        • Immunoglobulin heavy constant gamma 4
        • immunoglobulin heavy constant gamma 4 (G4m marker)
        • MGC117419
        • MGC45809
        see all

      • Relevance

        IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

      • Cellular localization

        Cell Membrane and Secreted

      Images

      • ELISA - Native Human IgG protein (ab98981)
        ELISA - Native Human IgG protein (ab98981)

        The wells were coated with ab195576 at 0.5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 40 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. ab201484 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (1)

      Publishing research using ab98981? Please let us know so that we can cite the reference in this datasheet.

      ab98981 has been referenced in 1 publication.

      • Gerlach D  et al. Methicillin-resistant Staphylococcus aureus alters cell wall glycosylation to evade immunity. Nature 563:705-709 (2018). PubMed: 30464342

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-7 of 7 Abreviews or Q&A

      Question

      I have two questions about three products. First, what is the difference between ab91102 and ab98981? Second, can ab168861 be used to detect ab91102 and ab98981 in western blot?

      Read More
      Answer

      These two IgG proteins are similar in many ways including origin, and levels of purity. However they do differ in regards to the storage buffer in which they are provided. ab98981: Preservative: None Constituents: 0.02M Ammonium bicarbonate (May contain traces of buffer salts) ab91102: Preservative: 0.05% Sodium Azide Constituents: 20mM PBS, 150mM Sodium chloride, pH 7.4. The Anti-Lambda Light chain [HP6054] antibody, ab168861, will react with any Lambda light chain which is present in these products and is guaranteed to work in WB, IHC-P, IHC-Fr. In reviewing the IgG proteins I do not see that these are kappa or lambda Light chain. The total kappa to lambda ratio is roughly 2:1 in serum and I would expect a similar ratio with these products.

      Read More

      Question

      The ELISA format is as follows We coat nunc-maxiSorp plate with 10ug/mL Human IgG (Abcam)ab98981. Next day block with standard 2%PBS/PBS. Wash plate pipet positive control (mouse anti-human IgG Purified) and subsequent samples. Samples are hybridoma supernatant resulting from murine hybridoma. Secondary antibody is goatxmouse IgG HRP. The problem we are having is the conjugate is binding to the plate yielding false positives, we can not attribute confidently the titer received from the hybridoma supe.

      Read More
      Answer

      I would recommend use of a secondary antibody against mouse IgG which has been pre-absorbed against human IgG. These antibodies are run through columns containing IgGs of many species and and antibody which reacts with those IgGs will be trapped in the columns. The two products I would recommend are: https://www.abcam.com/goat-fab2-polyclonal-secondary-antibody-to-mouse-igg-fc-hrp-pre-adsorbed-ab5879.html or https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg-h-l-hrp-pre-adsorbed-ab97040.html These antibodies each have less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected. They are tested and guaranteed in ELISA and are covered by our AbPromise.

      Read More

      Question

      Product code: 98981

      Inquiry: Hi, Is product ab98981 (Human IgG protein) suitable for cell culture use? Thanks for your help.

      Read More
      Answer

      Thank you for contacting us and I am sorry for the delay in getting back to you.

      I cannot see why the Human IgG protein (ab98981) could not be used in cell culture. However, I would bear in mind that the product has not been sterilized and may contaminate the cell culture and as we have not used it ourselves in this application ourselves we would not be able to guarantee its performance.

      I am sorry I could not be or more help in this instance. If you have any further questions, please do not hesitate to let me know.

      Read More

      Question

      Dear Sirs,

      I was wondering if you could let me know what are the main differences between your 2 products: ab91102 and ab98981?

      I look forward to hearing from you soon.

      Kind Regards,

      Read More
      Answer

      Thank you for your enquiry and your interest.

      These two products are from different sources and there are slight variations between them.

      ab91102 is from one patient, tested in SDS-PAGE and the storage buffer contains 0.05% Sodium Azide, 20mM PBS, 150mM Sodium chloride, pH 7.4.

      The other product ab98981 is purified from pooled normal Human serum/plasma, tested in RID and SDS-PAGE and the storage buffer contains 0.02M Ammonium bicarbonate (no preservative).

      I hope this helps and if I can assist further, please do not hesitate to contact me.

      Read More

      Question

      Reconstituted the protein and has stored it at 4C for 3 months. Is is still ok?

      Read More
      Answer

      Thank you for your patience in this matter.

      The storage at 2-8 is relevant to the freeze dried product. Once resuspended we would not really recommend storage at 2-8 for no more than 1-2 weeks, and this would depend on the diluent used. We would suggest aliquoting and storing frozen at <-15, preferable -80C for long term storage.

      If there is anything else I can help you with, please let me know.

      Read More

      Question

      Dear Sir/ Madam, Sorry to disturb you. Our customer advances an enquiry about ab98981(Human IgG protein) and ab7500 (Anti-Human IgG antibody [CH1]). She has purchases this two items and would like to know the Kd (Dissociation constant) between antigen (ab98981) and antibody (ab7500). Would you please help us provide the information? Thank you very much. Best regards,

      Read More
      Answer

      Thank you and your customer for your inquiry.

      Unfortunately, the Kd (Dissociation constant) of ab7500 was not experimentally tested and we cannot provide any data for this.

      But I can confirm that ab7500 is guaranteed for peptide ELISA with human samples by our Abpromise guarantee.

      I am sorry I could not provide more data on this occasion and hope this information will nevertheless be helpful for your customer.

      Read More

      Question

      Does the denaturing of the protein only occurs when the protein is treated wtih 6.0M urea or 6.0M guanidine hydrochloride? If the urea or guanidine hydrochloride is removed and another chemical substance is added, would the protein return to its un-denatured state?

      Read More
      Answer

      Thank you for your enquiry. The denaturing process is reversible in some cases. The original structure of some proteins can be regenerated by removal of the denaturing agent. I have attached a link to Wikipedia regarding protein denaturing if this will be helpful. Wikipedia: Denaturation (biochemistry) http://jp.adminsite.com/admin/abcamshare/CRM/attach/2011/10/17/CCE3215836_Denaturation(biochemistry)-Wikipedia_thefreeencyclopedia.mht

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com