For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    native-human-igg-protein-ab98981.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Immunoglobulins Heavy Chain IgG
Share by email

Native Human IgG protein (ab98981)

  • Datasheet
  • SDS
Submit a review Q&A (7)References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

ELISA - Native Human IgG protein (ab98981)

    Key features and details

    • Expression system: Native
    • Suitable for: RID, SDS-PAGE

    You may also be interested in

    ELISA
    Product image
    Human IgG ELISA Kit (ab195215)
    Primary
    Product image
    Anti-Human IgG antibody [IG266] (ab200699)
    Protein
    Native Human IgA protein (ab205803)

    View more associated products

    Description

    • Product name

      Native Human IgG protein
    • Purity

      96 % .
      IgA and IgM levels are <1% of the total IgG content by RID. ab98981 shows a single band corresponding to gamma electrophoretic mobility by Cellulose Acetate Electrophoresis.
    • Expression system

      Native
    • Accession

      P01857
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Native

      Associated products

      • Related Products

        • Anti-Human IgG antibody (ab2410)
        • FITC Anti-Human IgG antibody (ab4220)
        • Anti-Human IgG antibody [08C05] (ab72528)
        • FITC Anti-Human IgG antibody (ab81051)

      Specifications

      Our Abpromise guarantee covers the use of ab98981 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        Radial Immunodiffusion

        SDS-PAGE

      • Form

        Lyophilized
      • Additional notes

        Starting material serum donor tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies.

        IgG from pooled normal human serum/plasma

      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C.

        Constituent: 0.158% Ammonium bicarbonate

      • Reconstitution
        Reconstitution in phosphate buffer, pH >7.0 containing 0.15M NaCl is recommended.

      General Info

      • Alternative names

        • FLJ39988
        • FLJ40036
        • FLJ40253
        • FLJ40587
        • FLJ40789
        • FLJ40834
        • G1m marker
        • G2m marker
        • G3m marker
        • G4m marker
        • HDC
        • Heavy chain disease protein
        • Human immunglobulin G
        • Ig gamma 1 chain C region
        • Ig gamma 2 chain C region
        • Ig gamma 3 chain C region
        • Ig gamma 4 chain C region
        • IgG heavy chain locus
        • IGHG 1
        • IGHG 2
        • IGHG 3
        • IGHG 4
        • IGHG1
        • IGHG2
        • IGHG3
        • IGHG4
        • Immunoglobulin Gm1
        • Immunoglobulin Gm3
        • Immunoglobulin Gm4
        • Immunoglobulin heavy constant gamma 1
        • immunoglobulin heavy constant gamma 1 (G1m marker)
        • Immunoglobulin heavy constant gamma 2
        • immunoglobulin heavy constant gamma 2 (G2m marker)
        • Immunoglobulin heavy constant gamma 3
        • immunoglobulin heavy constant gamma 3 (G3m marker)
        • Immunoglobulin heavy constant gamma 4
        • immunoglobulin heavy constant gamma 4 (G4m marker)
        • MGC117419
        • MGC45809
        see all
      • Relevance

        IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
      • Cellular localization

        Cell Membrane and Secreted

      Images

      • ELISA - Native Human IgG protein (ab98981)
        ELISA - Native Human IgG protein (ab98981)

        The wells were coated with ab195576 at 0.5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 40 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. ab201484 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab98981? Please let us know so that we can cite the reference in this datasheet.

      ab98981 has been referenced in 1 publication.

      • Gerlach D  et al. Methicillin-resistant Staphylococcus aureus alters cell wall glycosylation to evade immunity. Nature 563:705-709 (2018). PubMed: 30464342

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-7 of 7 Abreviews or Q&A

      Question

      I have two questions about three products. First, what is the difference between ab91102 and ab98981? Second, can ab168861 be used to detect ab91102 and ab98981 in western blot?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 25 2013

      Answer

      These two IgG proteins are similar in many ways including origin, and levels of purity. However they do differ in regards to the storage buffer in which they are provided. ab98981: Preservative: None Constituents: 0.02M Ammonium bicarbonate (May contain traces of buffer salts) ab91102: Preservative: 0.05% Sodium Azide Constituents: 20mM PBS, 150mM Sodium chloride, pH 7.4. The Anti-Lambda Light chain [HP6054] antibody, ab168861, will react with any Lambda light chain which is present in these products and is guaranteed to work in WB, IHC-P, IHC-Fr. In reviewing the IgG proteins I do not see that these are kappa or lambda Light chain. The total kappa to lambda ratio is roughly 2:1 in serum and I would expect a similar ratio with these products.

      Read More

      Keith B

      Abcam Scientific Support

      Answered on Jun 25 2013

      Question

      The ELISA format is as follows We coat nunc-maxiSorp plate with 10ug/mL Human IgG (Abcam)ab98981. Next day block with standard 2%PBS/PBS. Wash plate pipet positive control (mouse anti-human IgG Purified) and subsequent samples. Samples are hybridoma supernatant resulting from murine hybridoma. Secondary antibody is goatxmouse IgG HRP. The problem we are having is the conjugate is binding to the plate yielding false positives, we can not attribute confidently the titer received from the hybridoma supe.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 26 2013

      Answer

      I would recommend use of a secondary antibody against mouse IgG which has been pre-absorbed against human IgG. These antibodies are run through columns containing IgGs of many species and and antibody which reacts with those IgGs will be trapped in the columns. The two products I would recommend are: https://www.abcam.com/goat-fab2-polyclonal-secondary-antibody-to-mouse-igg-fc-hrp-pre-adsorbed-ab5879.html or https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg-h-l-hrp-pre-adsorbed-ab97040.html These antibodies each have less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected. They are tested and guaranteed in ELISA and are covered by our AbPromise.

      Read More

      Abcam Scientific Support

      Answered on Apr 26 2013

      Question

      Product code: 98981

      Inquiry: Hi, Is product ab98981 (Human IgG protein) suitable for cell culture use? Thanks for your help.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 13 2012

      Answer

      Thank you for contacting us and I am sorry for the delay in getting back to you.

      I cannot see why the Human IgG protein (ab98981) could not be used in cell culture. However, I would bear in mind that the product has not been sterilized and may contaminate the cell culture and as we have not used it ourselves in this application ourselves we would not be able to guarantee its performance.

      I am sorry I could not be or more help in this instance. If you have any further questions, please do not hesitate to let me know.

      Read More

      Abcam Scientific Support

      Answered on Nov 13 2012

      Question

      Dear Sirs,

      I was wondering if you could let me know what are the main differences between your 2 products: ab91102 and ab98981?

      I look forward to hearing from you soon.

      Kind Regards,

      Read More

      Abcam community

      Verified customer

      Asked on Aug 16 2012

      Answer

      Thank you for your enquiry and your interest.

      These two products are from different sources and there are slight variations between them.

      ab91102 is from one patient, tested in SDS-PAGE and the storage buffer contains 0.05% Sodium Azide, 20mM PBS, 150mM Sodium chloride, pH 7.4.

      The other product ab98981 is purified from pooled normal Human serum/plasma, tested in RID and SDS-PAGE and the storage buffer contains 0.02M Ammonium bicarbonate (no preservative).

      I hope this helps and if I can assist further, please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on Aug 16 2012

      Question

      Reconstituted the protein and has stored it at 4C for 3 months. Is is still ok?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 21 2012

      Answer

      Thank you for your patience in this matter.

      The storage at 2-8 is relevant to the freeze dried product. Once resuspended we would not really recommend storage at 2-8 for no more than 1-2 weeks, and this would depend on the diluent used. We would suggest aliquoting and storing frozen at <-15, preferable -80C for long term storage.

      If there is anything else I can help you with, please let me know.

      Read More

      Abcam Scientific Support

      Answered on Jun 21 2012

      Question

      Dear Sir/ Madam, Sorry to disturb you. Our customer advances an enquiry about ab98981(Human IgG protein) and ab7500 (Anti-Human IgG antibody [CH1]). She has purchases this two items and would like to know the Kd (Dissociation constant) between antigen (ab98981) and antibody (ab7500). Would you please help us provide the information? Thank you very much. Best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Feb 10 2012

      Answer

      Thank you and your customer for your inquiry.

      Unfortunately, the Kd (Dissociation constant) of ab7500 was not experimentally tested and we cannot provide any data for this.

      But I can confirm that ab7500 is guaranteed for peptide ELISA with human samples by our Abpromise guarantee.

      I am sorry I could not provide more data on this occasion and hope this information will nevertheless be helpful for your customer.

      Read More

      Abcam Scientific Support

      Answered on Feb 10 2012

      Question

      Does the denaturing of the protein only occurs when the protein is treated wtih 6.0M urea or 6.0M guanidine hydrochloride? If the urea or guanidine hydrochloride is removed and another chemical substance is added, would the protein return to its un-denatured state?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 18 2011

      Answer

      Thank you for your enquiry. The denaturing process is reversible in some cases. The original structure of some proteins can be regenerated by removal of the denaturing agent. I have attached a link to Wikipedia regarding protein denaturing if this will be helpful. Wikipedia: Denaturation (biochemistry) http://jp.adminsite.com/admin/abcamshare/CRM/attach/2011/10/17/CCE3215836_Denaturation(biochemistry)-Wikipedia_thefreeencyclopedia.mht

      Read More

      Abcam Scientific Support

      Answered on Oct 18 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.