Native Human IgG protein (ab98981)

Submit a review Q&A (9)

Overview

  • Product name
    Native Human IgG protein
  • Protein length
    Full length protein

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence

Specifications

Our Abpromise guarantee covers the use of ab98981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Radial Immunodiffusion

    SDS-PAGE

  • Purity
    96 % .
    IgA and IgM levels are <1% of the total IgG content by RID. ab98981 shows a single band corresponding to gamma electrophoretic mobility by Cellulose Acetate Electrophoresis.
  • Form
    Lyophilised
  • Additional notes

    Starting material serum donor tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies.

    IgG from pooled normal human serum/plasma

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C.

    Constituent: 0.158% Ammonium bicarbonate

  • Reconstitution
    Reconstitution in phosphate buffer, pH >7.0 containing 0.15M NaCl is recommended.

General Info

  • Alternative names
    • FLJ39988
    • FLJ40036
    • FLJ40253
    • FLJ40587
    • FLJ40789
    • FLJ40834
    • G1m marker
    • G2m marker
    • G3m marker
    • G4m marker
    • HDC
    • Heavy chain disease protein
    • Human immunglobulin G
    • Ig gamma 1 chain C region
    • Ig gamma 2 chain C region
    • Ig gamma 3 chain C region
    • Ig gamma 4 chain C region
    • IgG heavy chain locus
    • IGHG 1
    • IGHG 2
    • IGHG 3
    • IGHG 4
    • IGHG1
    • IGHG2
    • IGHG3
    • IGHG4
    • Immunoglobulin Gm1
    • Immunoglobulin Gm3
    • Immunoglobulin Gm4
    • Immunoglobulin heavy constant gamma 1
    • immunoglobulin heavy constant gamma 1 (G1m marker)
    • Immunoglobulin heavy constant gamma 2
    • immunoglobulin heavy constant gamma 2 (G2m marker)
    • Immunoglobulin heavy constant gamma 3
    • immunoglobulin heavy constant gamma 3 (G3m marker)
    • Immunoglobulin heavy constant gamma 4
    • immunoglobulin heavy constant gamma 4 (G4m marker)
    • MGC117419
    • MGC45809
    see all
  • Relevance
    IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
  • Cellular localization
    Cell Membrane and Secreted

Images

  • ELISA - Native Human IgG protein (ab98981)
    ELISA - Native Human IgG protein (ab98981)

    The wells were coated with ab195576 at 0.5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 40 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. ab201484 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.

References

ab98981 has not yet been referenced specifically in any publications.

Publishing research using ab98981? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-9 of 9 Abreviews or Q&A

Question

I have two questions about three products. First, what is the difference between ab91102 and ab98981? Second, can ab168861 be used to detect ab91102 and ab98981 in western blot?

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Answer

These two IgG proteins are similar in many ways including origin, and levels of purity. However they do differ in regards to the storage buffer in which they are provided. ab98981: Preservative: None Constituents: 0.02M Ammonium bicarbonate (May contain traces of buffer salts) ab91102: Preservative: 0.05% Sodium Azide Constituents: 20mM PBS, 150mM Sodium chloride, pH 7.4. The Anti-Lambda Light chain [HP6054] antibody, ab168861, will react with any Lambda light chain which is present in these products and is guaranteed to work in WB, IHC-P, IHC-Fr. In reviewing the IgG proteins I do not see that these are kappa or lambda Light chain. The total kappa to lambda ratio is roughly 2:1 in serum and I would expect a similar ratio with these products.

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Question

The ELISA format is as follows We coat nunc-maxiSorp plate with 10ug/mL Human IgG (Abcam)ab98981. Next day block with standard 2%PBS/PBS. Wash plate pipet positive control (mouse anti-human IgG Purified) and subsequent samples. Samples are hybridoma supernatant resulting from murine hybridoma. Secondary antibody is goatxmouse IgG HRP. The problem we are having is the conjugate is binding to the plate yielding false positives, we can not attribute confidently the titer received from the hybridoma supe.

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Answer

I would recommend use of a secondary antibody against mouse IgG which has been pre-absorbed against human IgG. These antibodies are run through columns containing IgGs of many species and and antibody which reacts with those IgGs will be trapped in the columns. The two products I would recommend are: https://www.abcam.com/goat-fab2-polyclonal-secondary-antibody-to-mouse-igg-fc-hrp-pre-adsorbed-ab5879.html or https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg-h-l-hrp-pre-adsorbed-ab97040.html These antibodies each have less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected. They are tested and guaranteed in ELISA and are covered by our AbPromise.

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Question

Product code: 98981

Inquiry: Hi, Is product ab98981 (Human IgG protein) suitable for cell culture use? Thanks for your help.

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Answer

Thank you for contacting us and I am sorry for the delay in getting back to you.

I cannot see why the Human IgG protein (ab98981) could not be used in cell culture. However, I would bear in mind that the product has not been sterilized and may contaminate the cell culture and as we have not used it ourselves in this application ourselves we would not be able to guarantee its performance.

I am sorry I could not be or more help in this instance. If you have any further questions, please do not hesitate to let me know.

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Question

Dear Sirs,

I was wondering if you could let me know what are the main differences between your 2 products: ab91102 and ab98981?

I look forward to hearing from you soon.

Kind Regards,

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Answer

Thank you for your enquiry and your interest.

These two products are from different sources and there are slight variations between them.

ab91102 is from one patient, tested in SDS-PAGE and the storage buffer contains 0.05% Sodium Azide, 20mM PBS, 150mM Sodium chloride, pH 7.4.

The other product ab98981 is purified from pooled normal Human serum/plasma, tested in RID and SDS-PAGE and the storage buffer contains 0.02M Ammonium bicarbonate (no preservative).

I hope this helps and if I can assist further, please do not hesitate to contact me.

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Question

Reconstituted the protein and has stored it at 4C for 3 months. Is is still ok?

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Answer

Thank you for your patience in this matter.

The storage at 2-8 is relevant to the freeze dried product. Once resuspended we would not really recommend storage at 2-8 for no more than 1-2 weeks, and this would depend on the diluent used. We would suggest aliquoting and storing frozen at <-15, preferable -80C for long term storage.

If there is anything else I can help you with, please let me know.

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Question

Dear Sir/ Madam, Sorry to disturb you. Our customer advances an enquiry about ab98981(Human IgG protein) and ab7500 (Anti-Human IgG antibody [CH1]). She has purchases this two items and would like to know the Kd (Dissociation constant) between antigen (ab98981) and antibody (ab7500). Would you please help us provide the information? Thank you very much. Best regards,

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Answer

Thank you and your customer for your inquiry.

Unfortunately, the Kd (Dissociation constant) of ab7500 was not experimentally tested and we cannot provide any data for this.

But I can confirm that ab7500 is guaranteed for peptide ELISA with human samples by our Abpromise guarantee.

I am sorry I could not provide more data on this occasion and hope this information will nevertheless be helpful for your customer.

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Question

To whom it may concern: Our lab is preparing to perform a series of indirect ELISAs using human serum samples against a group of immunogenic proteins in an effort to gauge differential expression of antibodies (IgG1,2,3,4) directed against those proteins. We would like to perform standard curves using known concentrations of human Ig proteins and to test the efficiency of our secondaries direct against the Fc portions of human IgGs. Our thought was to use your product (Human IgG protein #ab98981) as a coating protein to be detected by our secondaries in parallel to wells containing the immunogen of interest. Would you advise this product for this purpose, or suggest an alternative?  Thank you for your assistance.

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Answer

Thank you for contacting us.

This product has not been used in ELISA and as such is not covered by our Abpromise guarantee in this assay. However, after reviewing your idea, I see no reason to believe that this product (ab91102) or our other Human IgG protein (ab98981) would not work.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Does the denaturing of the protein only occurs when the protein is treated wtih 6.0M urea or 6.0M guanidine hydrochloride? If the urea or guanidine hydrochloride is removed and another chemical substance is added, would the protein return to its un-denatured state?

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Answer

Thank you for your enquiry. The denaturing process is reversible in some cases. The original structure of some proteins can be regenerated by removal of the denaturing agent. I have attached a link to Wikipedia regarding protein denaturing if this will be helpful. Wikipedia: Denaturation (biochemistry) http://jp.adminsite.com/admin/abcamshare/CRM/attach/2011/10/17/CCE3215836_Denaturation(biochemistry)-Wikipedia_thefreeencyclopedia.mht

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Question

I am seeing several bands on my SDS-PAGE gel when I am staining with Coomassie blue ranging from 150-250 kDa (under non-reduced and non-denatured conitions). Can you tell me if there is anything else present in this protein that would leave bands at these molecular weights?   Thank you.

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Answer

I have contacted our lab on your behalf regarding the issues you are having with the IgG protein you bought from Abcam. Our lab personnel are requesting the lot number of the product you have so that they can test it and see if what else is present that could create the extra bands. Could you please get back to me with this information so that we can have some answers for you as soon as possible? I look forward to hearing back from you.

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