Key features and details
- Expression system: Native
- Suitable for: RID, SDS-PAGE
Product nameNative Human IgG protein
Purity96 % .
IgA and IgM levels are <1% of the total IgG content by RID. ab98981 shows a single band corresponding to gamma electrophoretic mobility by Cellulose Acetate Electrophoresis.
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab98981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Starting material serum donor tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies.
IgG from pooled normal human serum/plasma
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C.
Constituent: 0.158% Ammonium bicarbonate
ReconstitutionReconstitution in phosphate buffer, pH >7.0 containing 0.15M NaCl is recommended.
RelevanceIgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
Cellular localizationCell Membrane and Secreted
The wells were coated with ab195576 at 0.5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 40 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. ab201484 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab98981 has been referenced in 1 publication.
- Gerlach D et al. Methicillin-resistant Staphylococcus aureus alters cell wall glycosylation to evade immunity. Nature 563:705-709 (2018). PubMed: 30464342