Description

  • Product name

    Native Human IgG1 protein
    See all IgG1 proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Native
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human
    • Predicted molecular weight

      146 kDa

Specifications

Our Abpromise guarantee covers the use of ab90283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Protein Determination: Extinction Coeficient (E) 0.1% at 280nm =1.36.

    Prepared from myeloma plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.0536% PBS, 0.87% Sodium chloride

General Info

  • Alternative names

    • Ig gamma 1 chain C region
    • IgG heavy chain locus
    • IGHG1
    • Immunoglobulin Gm1
    • Immunoglobulin heavy constant gamma 1 (G1m marker)
    see all
  • Relevance

    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
  • Cellular localization

    Secreted. Cell membrane; Single-pass membrane protein

Images

  • The wells were coated with ab200699 at 5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG1 (ab90283) was then added starting at 100 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. Ab201485 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.

References

This product has been referenced in:

  • Vidal M  et al. Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens. J Immunol Methods N/A:N/A (2018). Read more (PubMed: 29397157) »
  • MacKay JL & Hammer DA Stiff substrates enhance monocytic cell capture through E-selectin but not P-selectin. Integr Biol (Camb) 8:62-72 (2016). Read more (PubMed: 26626697) »
See all 2 Publications for this product

Customer reviews and Q&As

1-10 of 16 Abreviews or Q&A

Question
Answer

Testing for glycosylation is not part of our standard QC and therefore we do not know the glycosylation status of this human IgG1.

We do not perform a deglycosylation step in our purification and so we expect IgG1 to be glycosylated but we cannot guarantee this as it has not been assessed.

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Answer

Neither of these proteins are recombinant. They are isolated from myeloma plasma, identified as IgG1 and IgG4 myelomas.

These products are tested for possible contaminating IgGs by ELISA. Each of these products is at least 95% pure and by ELISA are at least 95% true to subclass.

Read More

Answer

Tom is currently away from the office. Thus, I will be helping you. My name is Sybille.

As for ab18447, it is a protein G purified antibody that comes in PBS without any preservatives. As per datasheet, ab18447 is 'LEAF Purified'. This is a low endotoxin azide free formulation containing no preservative. The antibody is 0.2 µm filter sterilized and the endotoxin level is <0.01 ng/µg protein.

We have the human IgG1 protein (ab90283) that was purified human myeloma plasma and comes in 20mM PBS, 150mM Sodium chloride, pH 7.4 with 0.05% sodium azide. The nature of the light chain is kappa.

There would be no human raised monoclonal antibodies as antibodies are not raised in humans. Human antibodies are only purified from human blood/plasma.

Were you looking for a monoclonal antibody that is specific to a certain target?

Please let me know if I can help you in any other way.

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Answer

Thank you for contacting us.

The product ab90286 has kappa light chain however ab98893 has lamda light chain.

The proteins are dissolved in different storage buffer and the concentration is different.

These product are from human sources.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

The protein has a Kappa Light Chain.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your email.

I'm checking this with the lab and let you know what I find out.

Thank you for your patience!

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Answer

Thank you for your patience.

The lab did not have any further information regarding the sequence or potential sequence or post-translational differences between human individuals

But we do know there are 3 separate constant regions of the heavy chain and 1 found on the light chain.

Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

The information I have received so far from the lab is that they have not had this protein sequenced. They prepared the protein from the plasma of a single donor. It is not from a cell line.

I'm still checking with them regarding the UniProt database entry. But I think it is safe to assume that the sequence is identical or almost identical, except for the occasional point mutation.

I'll let you know if I receive more information.
Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us. That note on the COA refers to our "Abpromise" guarantee, which states:

"Regional variations to our Abpromise may apply to the following countries: China, Korea, Singapore, Malaysia, Taiwan and Thailand, which operate a 120 day guarantee. Please contact your regional office for further details."

https://www.abcam.com/index.html?pageconfig=abpromise

The length of the guarantee, which promises a replacement or credit or refund if the product does not work as specified on the datasheet, is not the same as the expiration date. If stored at -20C as recommended on the datasheets, we expect these proteins to remain effective for ELISA for at least five years, but this is not tested.

Read More

Answer

Thank you for your email.

I am happy to check these concentrations for you. Please find here below the answers:

IgG1 protein (ab90283) you will receive 1 mg at 3.41mg/ml, ¨so it will be in 293ul



IgG2 protein (ab90284) you will receive 1 mg at 2.2mg/ml, so it will be in 455ul



IgG3 protein (ab118426) you will receive 1 mg at 1.16mg/ml, so it will bein 862ul



IgG4 protein (ab90286) you will receive1mg at a lot dependent concentration, between 1.09-1.12mg/ml, which will be between 917ul and 893ul

I hope this information is helpful. Please do not hesitate to contact us again, should you need any additional information or should you have any questions.

I wish youa great day!

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1-10 of 16 Abreviews or Q&A

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