Description

  • Product name

    Native Human IgG2 protein
    See all IgG2 proteins and peptides
  • Biological activity

    Shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests.
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human
    • Additional sequence information

      Prepared from myeloma plasma.

Specifications

Our Abpromise guarantee covers the use of ab90284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Extinction Coefficient (E) 0.1% at 280nm =1.36.

    Shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests.  

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.0536% PBS, 0.87% Sodium chloride

General Info

  • Alternative names

    • Ig gamma-2 chain C region
    • IGHG2
    • immunoglobulin Gm2
    • immunoglobulin heavy constant gamma 2 (G2m marker)
    • immunoglobulin heavy constant, gamma 2
    see all
  • Relevance

    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). IgG2 is the only IgG subclass which passes through the placenta at a level generally lower than that found in the mother. A deficiency of IgG2 indicates a poor antibody response to bacterial polysaccharides and can lead to increased susceptibility to infections caused by encapsulated bacteria.
  • Cellular localization

    Secreted

Images

  • SDS PAGE analysis ab90284 under reduced/heated conditions.

    Lane 1: LMW Standard;

    Lane 2: 5µg ab90284;

    Lane 3 10µg ab90284;

    Lane 4 20µg ab90284.

     

     

References

This product has been referenced in:

  • Masat E  et al. Long-term exposure to Myozyme results in a decrease of anti-drug antibodies in late-onset Pompe disease patients. Sci Rep 6:36182 (2016). Read more (PubMed: 27812025) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer




I can confirm that the light chain of this IgG2 protein is the kappa light chain.


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Answer

Thank you for your email.

I am happy to check these concentrations for you. Please find here below the answers:

IgG1 protein (ab90283) you will receive 1 mg at 3.41mg/ml, ¨so it will be in 293ul



IgG2 protein (ab90284) you will receive 1 mg at 2.2mg/ml, so it will be in 455ul



IgG3 protein (ab118426) you will receive 1 mg at 1.16mg/ml, so it will bein 862ul



IgG4 protein (ab90286) you will receive1mg at a lot dependent concentration, between 1.09-1.12mg/ml, which will be between 917ul and 893ul

I hope this information is helpful. Please do not hesitate to contact us again, should you need any additional information or should you have any questions.

I wish youa great day!

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Question
Answer

Thank you for contacting us. Because this IgG2 was purified from human myeloma plasma, we do not know the exact sequence. The sequence of the C region of human IgG2 can be found on the UniProt database: http://www.uniprot.org/uniprot/P01859 I hope this helps, please let me know if you need any additional information.

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