Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
Product nameNative Human IgG3 protein
See all IgG3 proteins and peptides
Purity> 95 % SDS-PAGE.
ab118426 is prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Protein lengthFull length protein
Predicted molecular weight170 kDa
Our Abpromise guarantee covers the use of ab118426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.88% Sodium chloride, 0.214% disodium hydrogen phosphate, 0.059% sodium dihydrogen phosphate
RelevanceIgG3, like IgG1, activates complement and has an affinity for fc receptors; thus IgG1 and IgG3 are the more effective of the IgG subclasses. An IgG3 deficiency is associated with Wiscott-Aldrich disease, systemic lupus erythematosus, juvenile diabetes mellitus, and peridontal infections. IgG anti-viral antibodies are restricted to IgG subclasses 1 and 3, with IgG3 antibodies being the first to appear in response to infection.
SDS PAGE analysis of ab118426 under reducing conditions (lanes 2-4) and non-reducing conditions (lanes 5-7) on 4 - 12% Bis-Tris NuPAGE gel.
Lane 1: ladder.
Lane 2: 5 μg (reduced / heated)
Lane 3: 10 μg (reduced / heated)
Lane 4: 20 μg (reduced / heated)
Lane 5: 5 μg (non-reduced / no heat)
Lane 6: 10 μg (non-reduced / no heat)
Lane 7: 20 μg (non-reduced / no heat)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab118426 has been referenced in 3 publications.
- Henson D et al. Association Between ApoA-I (Apolipoprotein A-I) Immune Complexes and Adverse Cardiovascular Events-Brief Report. Arterioscler Thromb Vasc Biol 39:1884-1892 (2019). PubMed: 31315438
- Shiroishi M et al. Structure-function analyses of a stereotypic rheumatoid factor unravel the structural basis for germline-encoded antibody autoreactivity. J Biol Chem 293:7008-7016 (2018). PubMed: 29523691
- Sinkovits G et al. Concentration and Subclass Distribution of Anti-ADAMTS13 IgG Autoantibodies in Different Stages of Acquired Idiopathic Thrombotic Thrombocytopenic Purpura. Front Immunol 9:1646 (2018). PubMed: 30061898