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  1. Link

    native-human-igm-mu-chain-plasma-ab91106.pdf

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Immunology Immunoglobulins Heavy Chain IgM
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Native Human IgM mu chain (plasma) (ab91106)

  • Datasheet
  • SDS
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SDS-PAGE - Native Human IgM mu chain (plasma) (ab91106)

    Key features and details

    • Expression system: Native
    • Purity: > 95% SDS-PAGE
    • Suitable for: SDS-PAGE

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    Human IgM ELISA Kit (ab214568)

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    Description

    • Product name

      Native Human IgM mu chain (plasma)
      See all IgM proteins and peptides
    • Purity

      > 95 % SDS-PAGE.
      IgM (mu chain) is purified by reducing the disulfide bond connecting it to the IgM kappa and lambda light chains.
    • Expression system

      Native
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Native
      • Species

        Human
      • Predicted molecular weight

        75 kDa
      • Additional sequence information

        Purified Native Human IgM-mu-chain is from Human Plasma.
    • Description

      Native Human IgM protein

    Associated products

    • Related Products

      • Anti-IgM antibody, prediluted (ab75669)
      • HRP Anti-IgM antibody [CH2] (ab79524)

    Specifications

    Our Abpromise guarantee covers the use of ab91106 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Additional notes

      Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.33. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. No reaction by IEP to antisera to kappa or lambda.

       

      IgM heavy chain (mu chain) consists of 576 amino acids; there are five mu chains in each IgM molecule. IgM heavy chain is purified by reducing the disulfide bond connecting it to the IgM kappa and lambda light chains. IgM mu chain is more highly conserved across different species than is the heavy chain of IgG or IgA.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20ºC.

      Constituent: 6% Acetic acid

    • Reconstitution
      Reconstitute with 1.0 M acetic acid to the prelyophilization volume. Dilutions must be made in Acetic Acid.

    General Info

    • Alternative names

      • AGM1
      • Constant region of heavy chain of IgM
      • DKFZp686I15196
      • DKFZp686I15212
      • FLJ00385
      • Hepatitis B virus receptor binding protein
      • Ig mu chain C region
      • IGHM
      • IgM heavy chain constant region
      • Immunoglobulin heavy constant mu
      • Immunoglobulin mu chain
      • Imunoglobulin heavy chain
      • Imunoglobulin heavy chain constant region mu
      • Imunoglobulin heavy chain mu constant region
      • MGC104996
      • MGC52291
      • MU
      • VH
      see all
    • Relevance

      IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.
    • Cellular localization

      Isoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein.

    Images

    • SDS-PAGE - Native Human IgM mu chain (plasma) (ab91106)
      SDS-PAGE - Native Human IgM mu chain (plasma) (ab91106)
      SDS-PAGE: 12% Tris HCl gel
      Lane 1. 5 μg IgM (reduced/heated)
      Lane 2. 10 μg IgM (reduced/heated)
      Lane 2. 20 μg IgM (reduced/heated)
      Lane 3. 5 μg IgM (non-reduced/no heat)
      Lane 4. 10 μg IgM (non-reduced/no heat)
      Lane 5. 20 μg IgM (non-reduced/no heat)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab91106? Please let us know so that we can cite the reference in this datasheet.

    ab91106 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

     Thank you very much for your answer.  If only acetic acid can be used to dilute the solution, what can we do to process the solution for tryptic digestion of this product? (pH 8.5 is needed)  Thanks and regards, 

    Read More

    Abcam community

    Verified customer

    Asked on Sep 16 2011

    Answer

    Thank you for your email. We recommended acetic acid because it was suspended in acetic before it was lyophilized. However in case of tryptic digestion biological ph will be needed. So I can recommend choosing one of the following methods to reconstitute this protein; - Dissolve the lyophilized product in acetic acid (100 ul) and the add PBS to make desired concentration. - Dissolve in PBS; if it does not dissolve then add a solvent e.g. acetic acid or Aceto Nitrile - Dissolve in DMSO - Other products we have in catalogue were dissolved in Tris buffer with Sodium chloride so this buffer could also be an option. I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Sep 16 2011

    Question

    Hi,Thanks a lot for your kind reply. I want to further inquire some details about this product , i.e., IgM protein (Human) (ab91106).After it is reconstituted in 1M acetic acid, Can I directly dilute the resultant acidic solution with water OR Ammonium bicarbonate buffer (pH 8.5)? I am not sure if there will be any precipitation after the dilution. Thanks and regards, 

    Read More

    Abcam community

    Verified customer

    Asked on Sep 15 2011

    Answer

    Thank you for contacting us. The only dilutant we recommend is acetic acid. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Sep 15 2011

    Question

    Hi, I just bought one of your product, i.e., IgM protein (Human) (ab91106), and have some questions for it.  Q1: This product is lyophilised powder. What is the buffer (or solvent) to reconstitute this powder?  Q2: As for this product, I also found some information in your website as below, Concentration (2.41 mg/ml) Storage buffer   Preservative: None                        Constituents: 1M Acetic acid  What is the meaning of them when this product is lyophilised powder? Thanks and regards,  

    Read More

    Abcam community

    Verified customer

    Asked on Sep 14 2011

    Answer

    Thank you for contacting us and accept my apologies as lab spent more time to find this information then anticipated. The product can be reconstituted in 1M acetic acid. The product was in 1M acetic acid before it was lyophilized. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Sep 14 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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