Native Human IgM mu chain (plasma) (ab91106)
- Datasheet
- References
- Protocols
Description
-
Product name
Native Human IgM mu chain (plasma)
See all IgM proteins and peptides -
Purity
> 95 % SDS-PAGE.
IgM (mu chain) is purified by reducing the disulfide bond connecting it to the IgM kappa and lambda light chains. -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
-
Species
Human -
Predicted molecular weight
75 kDa -
Additional sequence information
Purified Native Human IgM-mu-chain is from Human Plasma.
-
-
Description
Native Human IgM protein
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab91106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Form
Lyophilised -
Additional notes
Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.33. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. No reaction by IEP to antisera to kappa or lambda.
IgM heavy chain (mu chain) consists of 576 amino acids; there are five mu chains in each IgM molecule. IgM heavy chain is purified by reducing the disulfide bond connecting it to the IgM kappa and lambda light chains. IgM mu chain is more highly conserved across different species than is the heavy chain of IgG or IgA.
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at -20ºC.
Constituent: 6% Acetic acid
-
ReconstitutionReconstitute with 1.0 M acetic acid to the prelyophilization volume. Dilutions must be made in Acetic Acid.
General Info
-
Alternative names
- AGM1
- Constant region of heavy chain of IgM
- DKFZp686I15196
see all -
Relevance
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated. -
Cellular localization
Isoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein.
Images
-
SDS-PAGE - Native Human IgM mu chain (plasma) (ab91106)SDS-PAGE: 12% Tris HCl gel
Lane 1. 5 μg IgM (reduced/heated)
Lane 2. 10 μg IgM (reduced/heated)
Lane 2. 20 μg IgM (reduced/heated)
Lane 3. 5 μg IgM (non-reduced/no heat)
Lane 4. 10 μg IgM (non-reduced/no heat)
Lane 5. 20 μg IgM (non-reduced/no heat)
References
ab91106 has not yet been referenced specifically in any publications.
