Description

  • Product name

    Native Human IgM mu chain (plasma)
    See all IgM proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    IgM (mu chain) is purified by reducing the disulfide bond connecting it to the IgM kappa and lambda light chains.
  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human
    • Predicted molecular weight

      75 kDa
    • Additional sequence information

      Purified Native Human IgM-mu-chain is from Human Plasma.
  • Description

    Native Human IgM protein

Specifications

Our Abpromise guarantee covers the use of ab91106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.33. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. No reaction by IEP to antisera to kappa or lambda.

     

    IgM heavy chain (mu chain) consists of 576 amino acids; there are five mu chains in each IgM molecule. IgM heavy chain is purified by reducing the disulfide bond connecting it to the IgM kappa and lambda light chains. IgM mu chain is more highly conserved across different species than is the heavy chain of IgG or IgA.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    Constituent: 6% Acetic acid

  • Reconstitution
    Reconstitute with 1.0 M acetic acid to the prelyophilization volume. Dilutions must be made in Acetic Acid.

General Info

  • Alternative names

    • AGM1
    • Constant region of heavy chain of IgM
    • DKFZp686I15196
    • DKFZp686I15212
    • FLJ00385
    • Hepatitis B virus receptor binding protein
    • Ig mu chain C region
    • IGHM
    • IgM heavy chain constant region
    • Immunoglobulin heavy constant mu
    • Immunoglobulin mu chain
    • Imunoglobulin heavy chain
    • Imunoglobulin heavy chain constant region mu
    • Imunoglobulin heavy chain mu constant region
    • MGC104996
    • MGC52291
    • MU
    • VH
    see all
  • Relevance

    IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.
  • Cellular localization

    Isoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein.

Images

  • SDS-PAGE: 12% Tris HCl gel
    Lane 1. 5 μg IgM (reduced/heated)
    Lane 2. 10 μg IgM (reduced/heated)
    Lane 2. 20 μg IgM (reduced/heated)
    Lane 3. 5 μg IgM (non-reduced/no heat)
    Lane 4. 10 μg IgM (non-reduced/no heat)
    Lane 5. 20 μg IgM (non-reduced/no heat)

References

ab91106 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your email. We recommended acetic acid because it was suspended in acetic before it was lyophilized. However in case of tryptic digestion biological ph will be needed. So I can recommend choosing one of the following methods to reconstitute this protein; - Dissolve the lyophilized product in acetic acid (100 ul) and the add PBS to make desired concentration. - Dissolve in PBS; if it does not dissolve then add a solvent e.g. acetic acid or Aceto Nitrile - Dissolve in DMSO - Other products we have in catalogue were dissolved in Tris buffer with Sodium chloride so this buffer could also be an option. I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

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Answer

Thank you for contacting us. The only dilutant we recommend is acetic acid. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us and accept my apologies as lab spent more time to find this information then anticipated. The product can be reconstituted in 1M acetic acid. The product was in 1M acetic acid before it was lyophilized. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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