Native human Lactate Dehydrogenase Isoenzyme V protein (ab77945)

Description

  • Product name

    Native human Lactate Dehydrogenase Isoenzyme V protein
  • Biological activity

    Specific Activity: 150 Units/mg protein. One unit will catalyze the transformation of one micromole of Lactate to pyruvate at 37°C and pH 8.55

  • Purity

    = 100 % Ammonium Sulphate Precipitation.
    ab77945 is 100% pure as determined by electrophoresis. Contaminants: ALP: 0.002%, GOT: 0.20%, GGT: <0.001%, GPT: 0.01%.
  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab77945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

    pH: 8.30
    Constituents: 0.0154% DTE (1,4-Dithioerythritol), 0.79% Tris HCl, 0.605% Tris, 0.0292% EDTA

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Cell proliferation-inducing gene 19 protein
    • L lactate dehydrogenase A chain
    • Lactate dehydrogenase A
    • LDH A
    • LDH M
    • LDH muscle subunit
    • LDH1
    • LDH5
    • LDHA
    • PIG 19
    • PIG19
    • Renal carcinoma antigen NY-REN-59
    see all
  • Relevance

    Lactate dehydrogenase 5 (LDH5) is one of five LDH isoenzymes and important for promoting anaerobic glycolysis. LDH5 is transcriptionally regulated by the hypoxia inducible factors (HIF) 1a and 2a.
  • Cellular localization

    Cytoplasmic

Images

References

ab77945 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question
Answer

Thank you for your phone call.
As we discussed I am sending you the 3 discount codes for the 2 antibodies as well as the one protein, all of which have not yet been tested in sandwich ELISA.
DISCOUNT CODE: xxx for ab85472
DISCOUNT CODE: xxx for ab101562
DISCOUNT CODE: xxx for ab77945
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab85472, ab101562, ab77945 in sandwich ELISA. Each code will give you either 1 free PRIMARY ANTIBODY or 1 FREE PROTEIN before the expiration date. To redeem this offer, please submit an Abreview for sandwich ELISA and include the codes in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your enquiry. Sorry for the confusing information appearing on the datasheet for ab77945. The actual concentration is 7.5mg/ml and the product activity is 1140 units/ml. I hope this is helpful. Please contact me again if you have any further questions.

Read More

Question
Answer

Here is the information specific for ab77945 Lot GR54583-2:    Activity:  1,140 Unit/ml @ 37°C Dimension Protein:  7.5 mg Protein/ml (Coomassie) Specific Activity: 150 Units/mg protein One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate at 37°C and pH 8.55   I hope this information is helpful.

Read More

Answer

Thanks for your inquiry. The new Lot GR54583-2 is at a higher concentration and therefore the 100 Unit package size will fit in a smaller vial compared to the previous lot. New Lot : concentration 1140 Units/mL = 88uL per 100 Units Old Lot: concentration 36 Units/mL = 2.78mL per 100 Units Both lots shoudl be labeled by abcam with the concentration showing "units/mL". The vial labels show "units/mL". The specification sheet that is included with each shipment shows both the "units/mL" activity and the "mg/mL" protein concentration. I hope this information is helpful. Please feel free to contact me if you require further assistance.

Read More

Question
Answer

Thanks for your call today and for letting us know about the problem with ab77945. As we discussed, I am sending a new vial which should arrive tomorrow. Please keep me updated about how this protein works in your assay. Also, if you have any data, please send it and I will see if there is a way for us to avoid this problem in the future. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up