Native human Lactate Dehydrogenase protein (ab77857)
Key features and details
- Expression system: Native
- Purity: >= 95% n/a
- Active: Yes
- Suitable for: WB, Functional Studies
Description
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Product name
Native human Lactate Dehydrogenase protein
See all Lactate Dehydrogenase proteins and peptides -
Biological activity
The activity (Units/mL) and specific activity (Units/mg) are unique to the lot, representative of how pure the lot is (higher purity = higher specific activity).
One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate per minute at 37°C and pH 9.4.
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Purity
>= 95 % n/a.
Purity is = 95% of LDH-1 as determined using Helena QuickGel® LD Isoenzyme Electrophoresis. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Additional sequence information
Source: Human Erythrocytes (Red Blood Cells)
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab77857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
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Form
Liquid -
Additional notes
Activity: 800 Units/mL (Dimension® Clinical Chemistry System)
Protein: 2.9 mg Protein/mL (Coomassie)
One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate per minute at 37°C and pH 9.4.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Constituents: 41% Ammonium sulphate, 0.15% DTT, 0.32% Tris HCl, 0.0292% EDTA
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Cell proliferation-inducing gene 19 protein
- GSD11
- L lactate dehydrogenase B chain
see all -
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
Involvement in disease
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. -
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab77857 has not yet been referenced specifically in any publications.