Native human Lactate Dehydrogenase protein (ab77857)

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Description

  • Product name

    Native human Lactate Dehydrogenase protein
    See all Lactate Dehydrogenase proteins and peptides

  • Biological activity

    The activity (Units/mL) and specific activity (Units/mg) are unique to the lot, representative of how pure the lot is (higher purity = higher specific activity).

    One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate per minute at 37°C and pH 9.4.

  • Purity

    >= 95 % n/a.
    Purity is = 95% of LDH-1 as determined using Helena QuickGel® LD Isoenzyme Electrophoresis.

  • Expression system

    Native

  • Accession

    P00338

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Native

    • Species

      Human

    • Additional sequence information

      Source: Human Erythrocytes (Red Blood Cells)

Specifications

Our Abpromise guarantee covers the use of ab77857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

  • Form

    Liquid

  • Additional notes

    Activity: 800 Units/mL (Dimension® Clinical Chemistry System)

    Protein: 2.9 mg Protein/mL (Coomassie)

    One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate per minute at 37°C and pH 9.4.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

    pH: 7.50
    Constituents: 41% Ammonium sulphate, 0.15% DTT, 0.32% Tris HCl, 0.0292% EDTA

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Cell proliferation-inducing gene 19 protein
    • GSD11
    • L lactate dehydrogenase B chain
    • L-lactate dehydrogenase A chain
    • Lactate dehydrogenase A
    • Lactate dehydrogenase B
    • Lactate dehydrogenase c variant 1
    • Lactate dehydrogenase c variant 3
    • Lactate dehydrogenase c variant 4
    • Lactate dehydrogenase C4
    • Lactate dehydrogenase H chain
    • Lactate dehydrogenase M
    • LDH A
    • LDH B
    • LDH H
    • LDH heart subunit
    • LDH M
    • LDH muscle subunit
    • LDH-A
    • LDH-M
    • LDH1
    • ldha
    • LDHA_HUMAN
    • LDHBD
    • LDHM
    • MS1111
    • PIG19
    • Proliferation inducing gene 19
    • Proliferation-inducing gene 19
    • Renal carcinoma antigen NY REN 46
    • Renal carcinoma antigen NY-REN-59
    • TRG 5
    • TRG5
    see all

  • Pathway

    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.

  • Involvement in disease

    Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.

  • Sequence similarities

    Belongs to the LDH/MDH superfamily. LDH family.

  • Post-translational
    modifications

    ISGylated.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

References

ab77857 has not yet been referenced specifically in any publications.

Publishing research using ab77857? Please let us know so that we can cite the reference in this datasheet.

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