Overview

  • Product name
    Native Human Laminin 5 protein
  • Protein length
    Full length protein

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab42326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

  • Purity
    >= 90 % SDS-PAGE.
    by affinity chromatography
  • Form
    Liquid
  • Additional notes

    Sourced from cell culture supernatant of Human foreskin keratinocytes. The protein is purified with an antibody against the beta 3 subunit. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 0.121% Sodium chloride, 0.88% Tris

General Info

  • Relevance
    Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature.

References

This product has been referenced in:
  • Jokela TA  et al. Microenvironment-Induced Non-sporadic Expression of the AXL and cKIT Receptors Are Related to Epithelial Plasticity and Drug Resistance. Front Cell Dev Biol 6:41 (2018). Read more (PubMed: 29719832) »
  • Li Y  et al. MMP-2 and MMP-13 affect vasculogenic mimicry formation in large cell lung cancer. J Cell Mol Med 21:3741-3751 (2017). Read more (PubMed: 28766880) »
See all 6 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

The Laminin 5 protein ab42326 was purified via affinity chromatography with an antibody specific to the beta3 chain. Therefore, it is likely that this Laminin is a mixture of the two splice variants Laminin-3A32 and Laminin-3B32. However, we have not confirmed this.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Answer

Thank you for contacting Abcam and for your interest in our Laminin 5 protein, ab42326.

At this time we have 15 units of this protein available between our US and UK offices. We do not expect this product to be backordered or for us to not maintain stock in the near future. You may also consider securing a supply of this protein by purchasing in bulk. Please contact mailto:sales@abcam.com for additional information regarding bulk options and pricing.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions or concerns.

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Question
Answer

Thank you for contacting us and your interest in our products.

I have looked into the Laminin 5 protein (ab42326) and whether it can be used for cell culture as you are hoping to do. Although we have not performed this experiment ourselves, I do not see why it could not be done. The ab42326 is the full length purified human laminin 5 protein purified from human foreskin keratinocytes. It should therefore be active. The attached journal makes references to how laminin can be used for cell culture which I hope may be of some use to you.

If you do choose to use the product for cell culture we would be very interested to know how you get on in order to let other cutomers know.

I hope this information has been of help. If you have any further questions please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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