Native Human Myoglobin protein (ab96036)

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SDS-PAGE - Native Human Myoglobin protein (ab96036)
  • HPLC - Native Human Myoglobin protein (ab96036)
  • Sandwich ELISA - Native Human Myoglobin protein (ab96036)

Key features and details

  • Expression system: Native
  • Purity: >= 96% n/a
  • Suitable for: SDS-PAGE, Immunodiffusion

Description

  • Product name

    Native Human Myoglobin protein
    See all Myoglobin proteins and peptides

  • Purity

    >= 96 % n/a.
    ab96036 is filtered through a 0.2µM membrane and 96% pure by SDS Page, Cellulose Acetate Electrophoresis and Radial immunodiffusion analyses.

  • Expression system

    Native

  • Accession

    P02144

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Native

    • Species

      Human

    • Predicted molecular weight

      17 kDa

    • Amino acids

      2 to 154

Specifications

Our Abpromise guarantee covers the use of ab96036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Immunodiffusion

  • Form

    Liquid

  • Additional notes

    Starting material donor tested and certified negative for HIV I and II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies. Product tested and found negative for HTLV antibodies.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    pH: 7.50
    Preservative: 0.09% Sodium azide
    Constituents: 0.05% PBS, 0.15% Sodium chloride

General Info

  • Alternative names

    • MB
    • MGC13548
    • MYG_HUMAN
    • Myoglobin

  • Function

    Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.

  • Sequence similarities

    Belongs to the globin family.
  • Information by UniProt

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Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab96036? Please let us know so that we can cite the reference in this datasheet.

ab96036 has been referenced in 1 publication.

  • Padmanaban G  et al. Identification of peptides that selectively bind to myoglobin by biopanning of phage displayed-peptide library. J Biotechnol 187C:43-50 (2014). ELISA . PubMed: 25078431

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Hi

I am looking for Myoglobin for HPLC analysis.

Can ab96036 be used as standard of HPLC analysis?

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Answer

Ab96036 is sold as a standard for the immunological determination of myoglobin in patients' blood serum samples, for example, after a suspected myocardial infarction. As batches are not routinely tested using size exclusion HPLC and consequently, it is not sold as an HPLC standard.

However, the purity of ab96036 is more than 96% which can be regarded as highly pure. Some batches were tested by size exclusion HPLC and a single peak of the expected molecular weight could be observed.

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Question

vielen Dank für ihre schnelle Antwort.

Vielleicht können sie mir noch einmal weiterhelfen. Enthalten die unten genannten Proteine noch ihr Eisen?



Mit freundlichen Grüßen,

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Answer



Ich freue mich Ihnen bestätigen zu können, dass ab53340 und ab96036 Eisen enthalten. Dies wurde von unserem Labor getestet und auch durch die Zugabe von Eisen im Herstellungs- und Aufreinigungsprozess sichergestellt.

Für ab103505 wurde der Eisengehalt leider nicht getestet und wir können deshalb keine Aussagen dazu machen.


Ich hoffe, diese Information ist hilfreich und wünsche ein schönes Wochenende.

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