Product nameNative human Neutrophil Elastase protein
See all Neutrophil Elastase proteins and peptides
Protein lengthFull length protein
Amino Acid Sequence
Molecular weight30 kDa
Our Abpromise guarantee covers the use of ab91099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityActivity: 20-22 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one micromole of Meo-suc-ala-pro-val-pNA per minute at 25°C in 100 mM Tris-HCl, pH 7.5, with 500 mM NaCl.
Purity> 95 % SDS-PAGE.
Additional notesReconstitute in 50 mM Na acetate pH 5.5 with 150 mM NaCl. Dissolve at a concentration of 1 to 2 mg/mL and store at -20 degrees C. If not immediately frozen, this enzyme should be used promptly to limit loss of activity. We do not suggest repeated thawing/freezing cycles. Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.985. Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti- HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20ºC.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Bone marrow serine protease
FunctionModifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
Tissue specificityBone marrow cells.
Involvement in diseaseDefects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain.
- Information by UniProt
This product has been referenced in:
- Wen G et al. Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29437605) »
- Thorlacius-Ussing J et al. Non-invasive profiling of protease-specific elastin turnover in lung cancer: biomarker potential. J Cancer Res Clin Oncol N/A:N/A (2018). Read more (PubMed: 30467633) »