Description

  • Product name

    Native human Plasmin protein
    See all Plasmin proteins and peptides
  • Biological activity

    Specific Activity: lot specifc. One unit is defined as the amount of enzyme that will hydrolyze 1 umole of tosyl-Gly-Pro-Lys-pNA per minute at 25°C, pH 7.8. One unit = 1.25 CU.

  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab84406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.

    pH: 7.30
    Constituents: 0.0131% EACA, 1.64% Sodium phosphate, 25% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Plasmin light chain B
    • Plasminogen
    • PLG
    • PLMN_HUMAN
  • Function

    Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
    Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
  • Tissue specificity

    Present in plasma and many other extracellular fluids. It is synthesized in the liver.
  • Involvement in disease

    Plasminogen deficiency
  • Sequence similarities

    Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 5 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Domain

    Kringle domains mediate interaction with CSPG4.
  • Post-translational
    modifications

    N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
    In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.
  • Cellular localization

    Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
  • Information by UniProt

References

ab84406 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Hello! I have contacted the laboratory and placed their reply below. Please let me know if you have any further questions.

How was the protein purified from plasma? – Answer from lab: We do not disclose out purification methods, but a quick internet search would reveal what is a well-known, established method for the purification of plasminogen. Here are a couple of links that I found regarding the purification of plasminogen -- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1243443/pdf/biochemj00806-0020.pdf



http://www.ncbi.nlm.nih.gov/pubmed/5475635

http://garfield.library.upenn.edu/classics1983/A1983RN13400001.pdf

and a book dealing with the subject: http://books.google.com/books/about/Human_plasminogen_purification_propertie.html?id=38pqAAAAMAAJ

Do you have suggestions on measuring plasmin? When I use the plate reader the color from the sample interferes with measurement. Answer from lab: We simply read optical density at 280nm and calculate protein concentration using the resulting absorbance and an extinction coefficient of 1.71 for a 0.1% solution. We use a Cary UV 100, which is a dual beam spectrophotometer for optical density readings. The lab suggestion is for them to perform a Bradford if a plate reader is their only option.

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Answer

Thank you for contacting Abcam regarding ab84406.


The activity for this protein (one unit) is defined as the amount of enzyme that will hydrolyze 1 umole of tosyl-Gly-Pro-Lys-pNA per minute at 25oC, pH 7.8. This assay is performed using 0.1 M Sodium Phosphate as the reaction buffer.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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