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How was this protein purified from plasma?
Do you have suggestions on measuring plasmin? when i use the plate reader the color from the sample intereferes with measurement?
Asked on Nov 05 2012
Hello! I have contacted the laboratory and placed their reply below. Please let me know if you have any further questions.
How was the protein purified from plasma? – Answer from lab: We do not disclose out purification methods, but a quick internet search would reveal what is a well-known, established method for the purification of plasminogen. Here are a couple of links that I found regarding the purification of plasminogen -- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1243443/pdf/biochemj00806-0020.pdf
and a book dealing with the subject: http://books.google.com/books/about/Human_plasminogen_purification_propertie.html?id=38pqAAAAMAAJ
Do you have suggestions on measuring plasmin? When I use the plate reader the color from the sample interferes with measurement. Answer from lab: We simply read optical density at 280nm and calculate protein concentration using the resulting absorbance and an extinction coefficient of 1.71 for a 0.1% solution. We use a Cary UV 100, which is a dual beam spectrophotometer for optical density readings. The lab suggestion is for them to perform a Bradford if a plate reader is their only option.
Answered on Nov 05 2012