Product nameNative human Plasmin protein (Active)
See all Plasmin proteins and peptides
15 units/mg protein
One unit is defined as the amount of enzyme that hydrolyzes one µmole of tosyl-Gly-Pro-Lys-pNA per minute at 25°C, pH 7.8. Note: One unit = 1.25 CU.
Purity>= 95 % SDS-PAGE.
Protein lengthFull length protein
Predicted molecular weight81 kDa
Our Abpromise guarantee covers the use of ab90928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Extinction Coeffcient (E) 0.1% at 280nm 1cm pathway =1.71
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20ºC.
Information available upon request.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionWe recommend that this product be resuspended in de-ionized H2O to the original volume.
- Plasmin light chain B
FunctionPlasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
Tissue specificityPresent in plasma and many other extracellular fluids. It is synthesized in the liver.
Involvement in diseasePlasminogen deficiency
Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
Contains 5 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain.
DomainKringle domains mediate interaction with CSPG4.
modificationsN-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.
Cellular localizationSecreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
- Information by UniProt
4-12% Bis-Tris NuPAGE gel.
Lane 1: Plasmin - 5 μg (reduced/ heated)
Lane 2: Plasmin - 10 μg (reduced/ heated)
Lane 3: Plasmin - 20 μg (reduced/ heated)
Lane 4: LMW Standard
Lane 5: Plasmin - 5 μg (non-reduced/ no heat)
Lane 6: Plasmin - 10 μg (non-reduced/ no heat)
Lane 7: Plasmin - 20 μg (non-reduced/ no heat)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab90928 has been referenced in 1 publication.
- Nishimura M et al. Extracellular cleavage of collagen XVII is essential for correct cutaneous basement membrane formation. Hum Mol Genet 25:328-39 (2016). PubMed: 26604146