Key features and details
- Expression system: Native
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameNative human Prethrombin 2 protein
Biological activityActivity was measured by clotting and/or chromogenic substrate assay, following conversion of prothrombin to thrombin.
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab62535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 50% Glycerol, 50% Water
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Prothrombin precursor
- Serine protease
RelevancePrethrombin 2, the smallest single-chain immediate precursor of a-thrombin (corresponding to residues Thr272 to Glu579 in prothrombin), has one glycosylation site at Asn373 and four disulfide bonds, Cys293-Cys439, Cys348-Cys364, Cys493-Cys507, and Cys521-Cys551. alpha-Thrombin can be derived from prothrombin by two pathways, each of which generates different intermediate products. In one pathway, human prothrombin is converted relatively slowly to alpha-thrombin in the presence of factor Xa and Ca2+ ions by initial cleavage at residue (Arg) 271/(Thr) 272 with consequent production of fragment 1.2 and prethrombin 2. Prethrombin 2 is then cleaved at R 320 with consequent generation of alpha-thrombin. In the other pathway, initial cleavage at R 320 yields an intermediate product, meizothrombin.
Cellular localizationSecreted, extra cellular space
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab62535 has been referenced in 1 publication.
- Jonebring A et al. Expression and characterization of recombinant ecarin. Protein J 31:353-8 (2012). PubMed: 22528138