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Native Human Prostate Specific Antigen protein (ab78528)

Reviews (1)Q&A (9)References (7)

Key features and details

  • Expression system: Native
  • Purity: >= 96% SDS-PAGE

Description

  • Product name

    Native Human Prostate Specific Antigen protein
    See all Prostate Specific Antigen proteins and peptides

  • Purity

    >= 96 % SDS-PAGE.
    ab78528 is 0.2µm filtered.

  • Expression system

    Native

  • Accession

    P07288

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Native

    • Species

      Human

    • Sequence

      MWVPVVFLTL SVTWIGAAPL ILSRIVGGWE CEKHSQPWQV LVASRGRAVC GGVLVHPQWV LTAAHCIRNK SVILLGRHSL FHPEDTGQVF QVSHSFPHPL YDMSLLKNRF LRPGDDSSHD LMLLRLSEPA ELTDAVKVMD LPTQEPALGT TCYASGWGSI EPEEFLTPKK LQCVDLHVIS NDVCAQVHPQ KVTKFMLCAG RWTGGKSTCS GDSGGPLVCN GVLQGITSWG SEPCALPERP SLYTKVVHYR KWIKDTIVAN P

Specifications

Our Abpromise guarantee covers the use of ab78528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

    Preservative: 0.09% Sodium azide
    Constituents: 0.134% PBS, 0.87% Sodium chloride

General Info

  • Alternative names

    • antigen, prostate-specific
    • APS
    • Gamma seminoprotein
    • Gamma-seminoprotein
    • hK3
    • Kallikrein 3
    • Kallikrein related peptidase 3
    • Kallikrein-3
    • KLK 3
    • KLK2A1
    • Klk3
    • KLK3_HUMAN
    • P-30 antigen
    • P30 antigen
    • Prostate-specific antigen
    • Psa
    • Semenogelase
    • Seminin
    see all

  • Function

    Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

  • Sequence similarities

    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (7)

Publishing research using ab78528? Please let us know so that we can cite the reference in this datasheet.

ab78528 has been referenced in 7 publications.

  • Hahn J  et al. Colorimetric switchable linker-based bioassay for ultrasensitive detection of prostate-specific antigen as a cancer biomarker. Analyst 144:4439-4446 (2019). PubMed: 31218301
  • Pernemalm M  et al. In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta. Elife 8:N/A (2019). PubMed: 30958262
  • Barbosa AI  et al. Towards One-Step Quantitation of Prostate-Specific Antigen (PSA) in Microfluidic Devices: Feasibility of Optical Detection with Nanoparticle Labels. Bionanoscience 7:718-726 (2017). PubMed: 29214121
  • Jazayeri MH  et al. Enhanced detection sensitivity of prostate-specific antigen via PSA-conjugated gold nanoparticles based on localized surface plasmon resonance: GNP-coated anti-PSA/LSPR as a novel approach for the identification of prostate anomalies. Cancer Gene Ther 23:365-369 (2016). PubMed: 27740614
  • Hughes AJ  et al. Microfluidic integration for automated targeted proteomic assays. Proc Natl Acad Sci U S A 109:5972-7 (2012). PubMed: 22474344
  • Berry SM  et al. Streamlining immunoassays with immiscible filtrations assisted by surface tension. Anal Chem 84:5518-23 (2012). PubMed: 22632629
  • Hughes AJ & Herr AE Microfluidic Western blotting. Proc Natl Acad Sci U S A : (2012). SDS-PAGE . PubMed: 23223527

Customer reviews and Q&As

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1-10 of 10 Abreviews or Q&A

Evaluation of the protease activity of PSA

 Excellent
Abreviews
abreview image
Application
ELISA
Evaluation of the protease activity of PSA.
Authors: Sofia Tellado Arbesú, Ana Díaz Fernández, María Jesús Lobo Castañón
Materials
PSA ab78528
Substrate: The peptide Mu-HSSKLQ-AMC, where Mu is 4-morpholine carbonyl and AMC represents 7-amino-4-methyl coumarin, was used as substrate.
Buffer: TBS, 50 mM Tris with 0.15 M NaCl, pH 7.6.
The substrate was dissolved in the TBS buffer at 1 mg/mL.

2. Protocol
To evaluate the protease activity of PSA over the selected substrate both reagents were mixed in the TBS buffer at room temperature in a final volume of 500 µL. The change in the fluorescence was monitored over time using a Perkin Elmer LS45 luminiscence spectrometer controlled with FL WinLab. The excitation and emission wavelengths were 355 nm and 460 nm, respectively. The cleavage of the peptide, catalyze by PSA, released AMC with a subsequent increase in the fluorescence at the selected wavelengths.

3. Results
A 0.5 µM solution of the substrate was incubated at room temperature with two different concentrations of PSA: 5 µg/mL and 15 µg/mL. We observed an increase of the fluorescence with time, at a highest rate for the highest PSA concentration (Figure 1). The peptide in the absence of PSA gives a stable value of fluorescence.
The enzymatic activity of PSA was further evaluated using a PSA concentration of 15 µg/mL and different concentrations of the substrate, in the range 0.1 to 1 µM. The change in fluorescence was monitored over time (Figure 2A). The initial rate for the hydrolysis reaction was obtained as the slope of the linear increase in fluorescence for each substrate concentration. The data were then fitted to the Michaelis-Menten equation to obtain the corresponding kinetic constants. Figure 2B shows the fitting curve. From this curve a Michaelis-Menten constant, Km, of 1,5 ± 0,9 µM was estimated.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

María Jesús Lobo

Verified customer

Submitted Dec 15 2020

Question

Can you please send me the PSA sample that you have ins stock ASAP (overnight). 

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Answer

Thank you for contacting us.

I am very sorry but I was mistaken when I earlier said that we had 1 vial remaining. I have been informed that this vial was removed from our system for testing as we investigate this matter. We are expecting a new lot but this could take 1-2 weeks to arrive. If you would like, I would be happy to send you a vial from the new lot free of charge. I do want you to know how very personally sorry I am about this and hope that we can resolve this for you.

I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

I was wondering if there is any information you have on the PSA samples, or if you will be sending me new samples soon.  

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Answer

Thank you for contacting us. I have been in contact with our supplier regarding this. They claim the product is not lyophilized. It seems that somewhere along the line these products may have opened or a crack may have appeared in the vial which may have led to leaking or evaporation of the product. We do have 1 more vial in stock, which I had our logistics team inspect to ensure that liquid was present. if you would like I can send you this vial free of charge. I have also created credit notes/refunds for orders xxxx and for xxxx. Your credit note IDs are xxxx and xxxx . I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice. Please let me know how you would like to proceed. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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Question

Customer kindly contacted us at this product has had less product than it should have when last received.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement added to the order number xxxx. This order now contains 1 free vial of ab78528 and one for which you have been charged. While we were not able to double check the volume in time for this order, we do want you to know that we will replace any product that does not have the full volume as stated on the datasheet. Please contact us if these new vials do not contain the full amount. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Question

Dear Sir,

I would like to get the quotation mentioned above in subject.

Also could you confirm the glycosylation on this protein?

Thanks a lot

Looking forward to hearing

Best Regards,

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Answer

Thank you for your enquiry which has been forwarded to the scientific support team. I apologize for the delay in obtaining the technical information for you, I have been waiting to hear back from the originator.

I can confirm that the ab78528 Prostate Specific Antigen proteinis not tested for glycosylation. The glycosylation pattern is expected to be similar to the glycosylation found in the donors, but this is not tested.

We aim to provide as much information as possible to customers and I am sorry there are no further details to send on this occasion. If you have any more questions, please do not hesitate to contact us.

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Question

Dear technical team,

Our customer purchased ab78528, ab403, ab53774 and ab9785.
His major is "electrical engineering' and he is going toapply those itemssimilar sELISA method.
He would like to confirm if PBS is okay as a dilution buffer.
Check this please.

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Answer

Thank you for contacting us.

Indeed, I can confirm that PBS as dilution buffer is suitable for all three antibodies and the protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I promised you some information about the antigen proteins before Christmas, and I could just make it, although I only had time to run an SDS-PAGE gel. So, I have attached here a picture of the gel. I also included in the gel the new CEA protein that I ordered. In the gel, CEA and PSA look fine, both have clear bands of the right size. There was also the right amount of protein solution in these tubes. However, MMP9 and MUC1 still do not look much better; MMP9 lane is practically empty and on the MUC1 lane, there are bands but nothing of the right size. I have loaded 1 µg of CEA, MUC1 and PSA, and 0.5 µg of MMP9. How do these proteins look like when you run them in a gel at Abcam? I have not used these proteins in my assays yet, ..... Thank you for your collaboration and merry Christmas! Best wishes,

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Answer

Thank you very much for the follow up. I am embarassed and very sorry for what has happened. It is most unfortunate that the new MMP9 protein also seems to have vanished. I will follow this up with the respective laboratory immediately. Indeed, for the MUC1 we had no choice than sending you the same batch. It does seem therefore to be a batch related problem - degradation/contamination problem. I will follow this up immediately as well. Unfortunately, being Christmas, I am not able to get the images/information now. I will however send them to you as soon as I can. Thank you for your patience and cooperation. I am very sorry and hope to clarify the problem as soon as possible. I am not sure what solution you would prefer, however be assured that we will recompensate those vials as well - either by refunding or replacing. Meanwhile, I wish you a merry Christmas as well and all the best for the new year with the new position!

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Question

Dear addressee, I ordered several cancer antigen proteins and antibodies from Abcam (xx) and received them a couple of weeks ago. I have checked them in an 12 % SDS-PAGE gel, and most of them are ok. However, there are problems with three of the cancer antigen proteins: 1) There is practically no protein in the MMP9 tube (see the SDS-PAGE picture attached). I have loaded 0.5 µg of this protein in the gel, but nothing is visible on the lane. So either the concentration of the MMP9 protein tube has been significantly lower than the data sheet indicates, or the protein has been degraded for some reason already on the way here (I have stored the protein in -20 C since the delivery, as recommended in the data sheet). I have also confirmed the result with a concentration measurement (Nanodrop spectrophotometer), which showed that there is very little if any protein. 2) There is also quite little protein on the MUC1 protein lane, and unfortunately, there is no single clear band which would represent the MUC1 protein. My concentration measurement (wtih Nanodrop) shows that there is protein in the tube, but according to the gel, it doesn't seem to be the right one. 3) PSA is fine according to this gel, the band is strong enough and there are only traces of contaminating proteins. However, when I opened the tube and pipetted from it for the first time (after spinning down the solution carefully), there was only 4 µl of PSA solution. According to my calculations, there should have been about 17 µl (the size of the batch was supposed to be 100 µg and the concentration 5.92 mg/ml, these values are from the tube label). I measured the concentration and it seems like the original concentration was somewhat higher than indicated in the tube, but not enough to compensate for the small volume. I also wonder if the protein has stayed native if the solution has dried out and been concentrated this significantly. I have attached here a picture of the SDS-PAGE gel to demonstrate the problem. I have loaded 1 µg of CEA, MUC1 and PSA, and 0.5 µg of MMP9. As you can see, CEA protein is immaculate. All antigens have been stored as recommended in the data sheets. I hope that you could find out what has gone wrong with these protein batches. I'm also asking if you could send new reagent tubes (at least of MMP9 and MUC1, which seem to lack the right protein completely, and are thus useless for us) free of charge, or if there is any other way of compensating for the missing proteins. I'm happy to give you more information about the batches if needed! Kind regards,

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Answer

Thank you for your email. We are very sorry to hear that you have received problematic protein vials. We do take product quality very seriously and will investigate how this could have had happened. We will obviously replace these proteins for you. For the PSA protein, we do have a other batch in stock and would be happy to send you a replacement vial of this batch if you would like. In regards to the MMP9 protein however, we do currently only have the same batch in stock. I would not want to send you a new vial of this batch before we do not have tested it on our side as well. Can we maybe send you an alternative product as replacement, for example, the his tagged MMP9s, ab82955, ab82907, ab82938 or the SDS ready MMP9 ab82957? Please let me know and I will arrange for sending out the replacement. For the MUC-1, we do also have only the same batch in stock. However in this case we do have no alternative product in the catalogue. Would you like to receive nevertheless a new vial of the same batch and see whether the new vial contains the entire product? Obviously, the new vial would also be covered by our Abpromise. Once again, please accept my apologies for the inconvenience. I am looking forward to hear back from you to know how to proceed.

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Question

We are interested in obtaining prostate specific antigen but are needing something with a known ratio with regards to free and complex PSA. Does this product contain both, and if so is the ratio set each time, or at least known for each batch? Many thanks

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Answer

Thank you for your enquiry. I can confirm that this product (ab78528: Prostate Specific Antigen protein) is free PSA and it does not contain complex PSA. This antigen is >98% pure. I hope this will be useful for you.

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Question

our user ordered ab78528. could you please let us know isoelectric point of this? if you do not test with this, please let us know sequence of this proteins. our user would like to calculate it in person. please check this for our user. thank you so much for your consideration all the time. I look forward to hearing from you.

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Answer

Thank you for your enquiry. The isoelectric point for this product has not been determined. Regarding the protein sequence, it is the full length native mature Prostate Specific Antigen (Swiss ID P07288). http://www.uniprot.org/uniprot/P07288 If there is anything else that I can help you with, please do not hesitate to contact me.

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