Product nameNative human Salivary alpha amylase protein
See all Salivary alpha amylase proteins and peptides
Activity is lot dependent. One unit will catalyze the hydrolysis of one micromole of malto pentaose, which through coupled reactions results in the formation of 5 micromoles of glucose-6-phosphate per minute at 37°C. Measured at 340nm as an equimolar amount of NADH produced by a coupled reaction.
Purity> 90 % SDS-PAGE.
Contaminants: Lipase: <0.001% Proteases: <0.001% Ammonia: <0.001 Umole/mg
Protein lengthFull length protein
SequenceMKLFWLLFTI GFCWAQYSSN TQQGRTSIVH LFEWRWVDIA LECERYLAPK GFGGVQVSPP NENVAIHNPF RPWWERYQPV SYKLCTRSGN EDEFRNMVTR CNNVGVRIYV DAVINHMCGN AVSAGTSSTC GSYFNPGSRD FPAVPYSGWD FNDGKCKTGS GDIENYNDAT QVRDCRLSGL LDLALGKDYV RSKIAEYMNH LIDIGVAGFR IDASKHMWPG DIKAILDKLH NLNSNWFPEG SKPFIYQEVI DLGGEPIKSS DYFGNGRVTE FKYGAKLGTV IRKWNGEKMS YLKNWGEGWG FMPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF TRVMSSYRWP RYFENGKDVN DWVGPPNDNG VTKEVTINPD TTCGNDWVCE HRWRQIRNMV NFRNVVDGQP FTNWYDNGSN QVAFGRGNRG FIVFNNDDWT FSLTLQTGLP AGTYCDVISG DKINGNCTGI KIYVSDDGKA HFSISNSAED PFIAIHAESK L
Our Abpromise guarantee covers the use of ab77875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 0.0111% Calcium chloride, 0.242% Tris, 0.87% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with Tris saline or other buffer to desired concentration. Lyophilized from 150mM Sodium chloride, 20mM Tris, 1mM Calcium chloride, pH 7.2. Protein: 0.40mg Protein/mg powder (Coomassie).
- amylase, salivary, B
- 1 4 alpha D glucan glucanohydrolase
Sequence similaritiesBelongs to the glycosyl hydrolase 13 family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab77875 has been referenced in 2 publications.
- Contreras-Aguilar MD et al. Variation of human salivary alpha-amylase proteoforms in three stimulation models. Clin Oral Investig N/A:N/A (2019). PubMed: 31388762
- Contreras-Aguilar MD et al. Detection and measurement of alpha-amylase in canine saliva and changes after an experimentally induced sympathetic activation. BMC Vet Res 13:266 (2017). PubMed: 28830550