Key features and details
- Expression system: Native
- Suitable for: Dot blot, ELISA, Immunomicroscopy, WB
Product nameNative Human Serum Albumin protein (Biotin)
See all Human Serum Albumin proteins and peptides
Protein lengthFull length protein
Predicted molecular weight69 kDa
DescriptionNative Human Human Serum Albumin protein (Biotin)
Our Abpromise guarantee covers the use of ab8033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
This product was prepared from normal serum delipidation a multi-stage process including selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human Albumin and anti-Human Serum.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
ReconstitutionRestore with 1.0 ml of deionized water (or equivalent).aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. Stabiliser: 10 mg/ml Polyethylene Glycol (PEG-8000) Preservative: 0.01% (w/v) Sodium Azide
FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Involvement in diseaseDefects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid.
- Information by UniProt
Dot Blot - Native Human Serum Albumin protein (Biotin) (ab8033).
A three-fold serial dilution of ab8033 starting at 200ng was spotted onto 0.45 µm nitrocellulose and blocked in 1% BSA-TTBS for 30 min at 20°C. An HRP conjugated Streptavidin was incubated at 1/40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab8033 has not yet been referenced specifically in any publications.