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Active full length protein
How is the protein prepared? Is this native purified protein?
Cleaved protein is active which would not be full length.
Asked on Jun 19 2015
I can confirm this is native fully active protein. The Prothrombin is purified directly from plasma. The Prothrombin is then activated to Thrombin via a Prothrombinase reaction.
PROTEOLYTIC ACTIVATION OF PROTHROMBIN The serine protease α-thrombin is produced by proteolytic activation of the zymogen, prothrombin. The enzyme complex, prothrombinase, catalyzes the proteolysis of two peptide bonds in prothrombin, which gives rise to an NH2-terminal derived F1.2 region and the heterodimer, alpha-thrombin. Alpha-thrombin is composed of an A chain (Mr=6000) which is covalently linked to a B chain (Mr=31,000) through a single disulfide bond.
I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.
Sam WasherAbcam Scientific Support
Answered on Jun 19 2015