Description

  • Product name

    Native human Trypsin protein
    See all Trypsin proteins and peptides
  • Biological activity

    Activity: >2.5units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of N-Benzyl-L-arginine-pNA per minute at 25°C in 200mM Tris-HCl, pH 7.8, 20mM CaCl2.
  • Purity

    > 95 % SDS-PAGE.
    ab78527 shows no chymotrypsin activity when assayed against N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-p-nitroanilide.
  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab78527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    Preservative: None

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in 93.5µl 2mM HCl. Use product immediately after bringing to neutral pH to avoid auto-degradation. After reconstitution, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.

General Info

  • Alternative names

    • Alpha-trypsin chain 2
    • Anionic trypsinogen
    • Beta trypsin
    • Beta-trypsin
    • Brain trypsinogen
    • Cationic trypsinogen
    • Digestive zymogen
    • Mesotrypsin
    • Mesotrypsinogen
    • MGC111183
    • MGC120174
    • MGC120175
    • MGC149362
    • MTG
    • Nonfunctional trypsin 1
    • Pancreatic trypsinogen III
    • Protease Serine 1
    • Protease serine 1 (trypsin 1)
    • Protease serine 2
    • Protease serine 2 (trypsin 2)
    • Protease serine 2 preproprotein
    • Protease serine 3
    • Protease serine 3 (mesotrypsin)
    • Protease serine 4 (trypsin 4 brain)
    • Prss1
    • PRSS2
    • PRSS3
    • PRSS4
    • Serine protease 1
    • Serine protease 2
    • Serine protease 3
    • Serine protease 4
    • T9
    • TCR V beta 4.1
    • TRP1
    • TRY1
    • TRY1_HUMAN
    • TRY2
    • TRY3
    • TRY4
    • TRY8
    • TRYP1
    • TRYP2
    • Trypsin 1
    • Trypsin 2
    • Trypsin 3
    • Trypsin I
    • Trypsin II
    • Trypsin III
    • Trypsin IV
    • Trypsinogen 1
    • Trypsinogen 2
    • Trypsinogen 4
    • Trypsinogen 5
    • Trypsinogen A
    • Trypsinogen IV
    • Trypsinogen, anionic
    • Trypsinogen, cationic
    see all
  • Function

    Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
  • Involvement in disease

    Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

Images

References

ab78527 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry.

Trypsin is a protease enzyme, and the ab78527Trypsinprotein has been tested as active. If the fluoresent protein the customer wishes to use is known to contain one of thesubstrate sequencesof the trypsin enzyme, then it may work:

Trypsin substrates:
Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec.

However, I am sorry to confirm that this protein has has never been tested in this type of application. All tested applications that we can guaranteeare specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

If the customer would like to try the product, we like to encourage all of our customers to submit an Abreview via the online product datasheet, and the customer may like to consider this. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I am sorry we do not have a trypsin product tested for the customers requirements on this occasion. However, I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

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