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Dear technical team,
Our customer would like to purchase ab78527 and wants to confirm it is right for his application.
He is going to treat ab78527 to substrate-fluorescent dye complex as a pretease.
If ab78527 break the amino acids bonds then it can be detected flurescence.
It it possible to use?
Check this for our customer.
Asked on Feb 14 2012
Thank you for your enquiry.
Trypsin is a protease enzyme, and the ab78527Trypsinprotein has been tested as active. If the fluoresent protein the customer wishes to use is known to contain one of thesubstrate sequencesof the trypsin enzyme, then it may work:
Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec.
However, I am sorry to confirm that this protein has has never been tested in this type of application. All tested applications that we can guaranteeare specified on our datasheets, and these are updated as soon as any new information is brought to our attention.
If the customer would like to try the product, we like to encourage all of our customers to submit an Abreview via the online product datasheet, and the customer may like to consider this. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.
To find out more about our Abreview system, please see the following link:
I am sorry we do not have a trypsin product tested for the customers requirements on this occasion. However, I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.
Answered on Feb 14 2012