Native Mouse Fibrinogen protein (Active) (ab92791)

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Description

  • Product name

    Native Mouse Fibrinogen protein (Active)
    See all Fibrinogen proteins and peptides

  • Biological activity

    >80% Clottable in a thrombin based assay.

  • Purity

    > 95 % SDS-PAGE.
    Prepared from fresh Mouse plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography.

  • Expression system

    Native

  • Accession

    E9PV24
    Q8K0E8
    Q8VCM7

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Native

    • Amino Acid Sequence 1

    • Sequence

      MLSLRVTCLI LSVASTVWTT DTEDKGEFLS EGGGVRGPRV VERHQSQCKD SDWPFCSDDD WNHKCPSGCR MKGLIDEANQ DFTNRINKLK NSLFDFQRNN KDSNSLTRNI MEYLRGDFAN ANNFDNTYGQ VSEDLRRRIE ILRRKVIEKA QQIQALQSNV RAQLIDMKRL EVDIDIKIRS CKGSCSRAVN REINLQDYEG HQKQLQQVIA KELLPTKDRQ YLPALKMSPV PDLVPGSFKS QLQEAPPEWK ALTEMRQMRM ELERPGKDGG SRGDSPGDSR GDSRGDFATR GPGSKAENPT NPGPGGSGYW RPGNSGSGSD GNRNPGTTGS DGTGDWGTGS PRPGSDSGNF RPANPNWGVF SEFGDSSSPA TRKEYHTGKA VTSKGDKELL IGKEKVTSSG TSTTHRSCSK TITKTVTGPD GRREVVKEVI TSDDGSDCGD ATELDISHSF SGSLDELSER HPDLSGFFDN HFGLISPNFK EFGSKTHSDS DILTNIEDPS SHVPEFSSSS KTSTVKKQVT KTYKMADEAG SEAHREGETR NTKRGRARAR PTRDCDDVLQ TQTSGAQNGI FSIKPPGSSK VFSVYCDQET SLGGWLLIQQ RMDGSLNFNR TWQDYKRGFG SLNDKGEGEF WLGNDYLHLL TLRGSVLRVE LEDWAGKEAY AEYHFRVGSE AEGYALQVSS YRGTAGDALV QGSVEEGTEY TSHSNMQFST FDRDADQWEE NCAEVYGGGW WYNSCQAANL NGIYYPGGTY DPRNNSPYEI ENGVVWVPFR GADYSLRAVR MKIRPLVGQ

    • Amino acids

      1 to 789

    • Additional sequence information

      Alpha chain Gene ID: 14161

    • Amino Acid Sequence 2

    • Sequence

      MRHLWLLLLL CVFSVQTQAA DDDYDEPTDS LDARGHRPVD RRKEEPPSLR PAPPPISGGG YRARPAKATA NQKKVERRPP DAGGCLHADT DMGVLCPTGC TLQQTLLNQE RPIKSSIAEL NNNIQSVSDT SSVTFQYLTL LKDMWKKKQA QVKENENVIN EYSSILEDQR LYIDETVNDN IPLNLRVLRS ILEDLRSKIQ KLESDISAQM EYCRTPCTVS CNIPVVSGKE CEEIIRKGGE TSEMYLIQPD TSIKPYRVYC DMKTENGGWT VIQNRQDGSV DFGRKWDPYK KGFGNIATNE DAKKYCGLPG EYWLGNDKIS QLTRMGPTEL LIEMEDWKGD KVKAHYGGFT VQNEASKYQV SVNKYKGTAG NALMDGASQL VGENRTMTIH NGMFFSTYDR DNDGWVTTDP RKQCSKEDGG GWWYNRCHAA NPNGRYYWGG LYSWDMSKHG TDDGVVWMNW KGSWYSMRRM SMKIRPFFPQ Q

    • Amino acids

      1 to 481

    • Additional sequence information

      Gene ID: 110135 Beta chain.

    • Amino Acid Sequence 3

    • Sequence

      MSWSLQPPSF LLCCLLLLFS PTGLAYVATR DNCCILDERF GSFCPTTCGI ADFLSSYQTD VDNDLRTLED ILFRAENRTT EAKELIKAIQ VYYNPDQPPK PGMIDSATQK SKKMVEEIVK YEALLLTHET SIRYLQEIYN SNNQKITNLK QKVAQLEAQC QEPCKDSVQI HDTTGKDCQE IANKGAKESG LYFIRPLKAK QQFLVYCEID GSGNGWTVLQ KRIDGSLDFK KNWIQYKEGF GHLSPTGTTE FWLGNEKIHL ISMQSTIPYA LRIQLKDWNG RTSTADYAMF RVGPESDKYR LTYAYFIGGD AGDAFDGYDF GDDPSDKFFT SHNGMQFSTW DNDNDKFEGN CAEQDGSGWW MNKCHAGHLN GVYHQGGTYS KSSTTNGFDD GIIWATWKSR WYSMKETTMK IIPFNRLSIG EGQQHHMGGS KQAGDV

    • Amino acids

      1 to 436

    • Additional sequence information

      Gene ID: 99571 Gamma chain.

  • Description

    Native Fibrinogen protein (Active)

Specifications

Our Abpromise guarantee covers the use of ab92791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid

  • Additional notes

    Extinction coefficient: 1.51

    Host species: Mouse

    MW: 340.00 kDa

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituent: 0.59% Sodium citrate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • FGA
    • FGB
    • FGG
    • Fib2
    • FIBA_HUMAN
    • Fibrinogen A alpha polypeptide
    • Fibrinogen alpha chain
    • Fibrinogen B alpha polypeptide
    • Fibrinogen beta chain
    • Fibrinogen G alpha polypeptide
    • Fibrinogen gamma chain
    • fibrinogen, B beta polypeptide
    • fibrinogen, G gamma polypeptide
    • fibrinogen, gamma polypeptide
    • Fibrinogen--alpha -polypeptide chain
    • Fibrinogen--beta -polypeptide chain
    • Fibrinogen--gamma-polypeptide chain
    see all

  • Function

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

  • Tissue specificity

    Plasma.

  • Involvement in disease

    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.

  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.

  • Domain

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

  • Post-translational
    modifications

    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.

  • Cellular localization

    Secreted.
  • Information by UniProt

References

This product has been referenced in:

  • Waffarn EE  et al. Infection-induced type I interferons activate CD11b on B-1 cells for subsequent lymph node accumulation. Nat Commun 6:8991 (2015). Read more (PubMed: 26612263) »
See 1 Publication for this product

Publishing research using ab92791? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

This customer raised an enquire about ab92791 (Fibrinogen protein (Active)), he wants to confirm is this antibody can specify recognize to the beta chain of fibrinogen protein but not alpha and gamma chain, could you please help this customer to double check this information? Thanks for your kindly assistance. Best regards

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Answer

Thank you for contacting us.
Please be advised that ab92791 is not an antibody; it is a protein that can be used with antibodies as positive control or can be used in functional assays.

I would suggest choosingan anti Fibrogen antibody from the list

https://www.abcam.com/index.html?pageconfig=searchresults&startPage=1
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Thanks for your kindly reply, this customer wants to know whether this protein through the deglycosylation after purification?

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Answer

Thank you for your reply. As these are purified native proteins, it is highly likely that they will be glycosylated. Please be aware that the proteins recommended are derived from different species: ab62394 Human ab92791 Mouse ab92827 Bovine ab92828 Pig ab93072 Rat Please do not hesitate to contact me should you have further questions.

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Question

Our customer raises an enquiry about ab92828 (Fibrinogen protein). He wants to access the efficacy and protein digested ability of their self-made endoglycosidase enzyme. Therefore, he purposes to use the endoglycosidase to interact with ab92828 then run SDS-PAGE to observe whether the digested fibrinogen protein with different molecular size could be detected and therefore confirm the enzyme activity. Thus he wants to know whether ab92828 can be used in this experiment. Could you please help this customer solve the problem? Thanks for your kindly help.

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Answer

Thank you for your reply. This product may be suitable, however considering the type of experiment the customer is looking to perform I would suggest using a protein that has been tested in a functional assay. This will ensure that the protein formulation does not contain reagents that will inhibit any reaction. Here is a list of potential products: ab62394 Fibrinogen protein Click here (or use the following: ab92791 Fibrinogen protein (Active) Click here (or use the following: ab92827 Fibrinogen protein (Active) Click here (or use the following: ab93072 Fibrinogen protein (Active) Click here (or use the following: Please do not hesitate to contact me should you have future questions.

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