Native Mouse Fibrinogen protein (Active) (ab92791)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Native Mouse Fibrinogen protein (Active)
See all Fibrinogen proteins and peptides -
Biological activity
>80% Clottable in a thrombin based assay.
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Purity
> 95 % SDS-PAGE.
Prepared from fresh Mouse plasma using several chromatographic steps. Plasminogen depleted by lysine affinity chromatography. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Amino Acid Sequence 1
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Sequence
MLSLRVTCLI LSVASTVWTT DTEDKGEFLS EGGGVRGPRV VERHQSQCKD SDWPFCSDDD WNHKCPSGCR MKGLIDEANQ DFTNRINKLK NSLFDFQRNN KDSNSLTRNI MEYLRGDFAN ANNFDNTYGQ VSEDLRRRIE ILRRKVIEKA QQIQALQSNV RAQLIDMKRL EVDIDIKIRS CKGSCSRAVN REINLQDYEG HQKQLQQVIA KELLPTKDRQ YLPALKMSPV PDLVPGSFKS QLQEAPPEWK ALTEMRQMRM ELERPGKDGG SRGDSPGDSR GDSRGDFATR GPGSKAENPT NPGPGGSGYW RPGNSGSGSD GNRNPGTTGS DGTGDWGTGS PRPGSDSGNF RPANPNWGVF SEFGDSSSPA TRKEYHTGKA VTSKGDKELL IGKEKVTSSG TSTTHRSCSK TITKTVTGPD GRREVVKEVI TSDDGSDCGD ATELDISHSF SGSLDELSER HPDLSGFFDN HFGLISPNFK EFGSKTHSDS DILTNIEDPS SHVPEFSSSS KTSTVKKQVT KTYKMADEAG SEAHREGETR NTKRGRARAR PTRDCDDVLQ TQTSGAQNGI FSIKPPGSSK VFSVYCDQET SLGGWLLIQQ RMDGSLNFNR TWQDYKRGFG SLNDKGEGEF WLGNDYLHLL TLRGSVLRVE LEDWAGKEAY AEYHFRVGSE AEGYALQVSS YRGTAGDALV QGSVEEGTEY TSHSNMQFST FDRDADQWEE NCAEVYGGGW WYNSCQAANL NGIYYPGGTY DPRNNSPYEI ENGVVWVPFR GADYSLRAVR MKIRPLVGQ -
Amino acids
1 to 789 -
Additional sequence information
Alpha chain Gene ID: 14161
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Amino Acid Sequence 2
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Sequence
MRHLWLLLLL CVFSVQTQAA DDDYDEPTDS LDARGHRPVD RRKEEPPSLR PAPPPISGGG YRARPAKATA NQKKVERRPP DAGGCLHADT DMGVLCPTGC TLQQTLLNQE RPIKSSIAEL NNNIQSVSDT SSVTFQYLTL LKDMWKKKQA QVKENENVIN EYSSILEDQR LYIDETVNDN IPLNLRVLRS ILEDLRSKIQ KLESDISAQM EYCRTPCTVS CNIPVVSGKE CEEIIRKGGE TSEMYLIQPD TSIKPYRVYC DMKTENGGWT VIQNRQDGSV DFGRKWDPYK KGFGNIATNE DAKKYCGLPG EYWLGNDKIS QLTRMGPTEL LIEMEDWKGD KVKAHYGGFT VQNEASKYQV SVNKYKGTAG NALMDGASQL VGENRTMTIH NGMFFSTYDR DNDGWVTTDP RKQCSKEDGG GWWYNRCHAA NPNGRYYWGG LYSWDMSKHG TDDGVVWMNW KGSWYSMRRM SMKIRPFFPQ Q -
Amino acids
1 to 481 -
Additional sequence information
Gene ID: 110135 Beta chain.
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Amino Acid Sequence 3
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Sequence
MSWSLQPPSF LLCCLLLLFS PTGLAYVATR DNCCILDERF GSFCPTTCGI ADFLSSYQTD VDNDLRTLED ILFRAENRTT EAKELIKAIQ VYYNPDQPPK PGMIDSATQK SKKMVEEIVK YEALLLTHET SIRYLQEIYN SNNQKITNLK QKVAQLEAQC QEPCKDSVQI HDTTGKDCQE IANKGAKESG LYFIRPLKAK QQFLVYCEID GSGNGWTVLQ KRIDGSLDFK KNWIQYKEGF GHLSPTGTTE FWLGNEKIHL ISMQSTIPYA LRIQLKDWNG RTSTADYAMF RVGPESDKYR LTYAYFIGGD AGDAFDGYDF GDDPSDKFFT SHNGMQFSTW DNDNDKFEGN CAEQDGSGWW MNKCHAGHLN GVYHQGGTYS KSSTTNGFDD GIIWATWKSR WYSMKETTMK IIPFNRLSIG EGQQHHMGGS KQAGDV -
Amino acids
1 to 436 -
Additional sequence information
Gene ID: 99571 Gamma chain.
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Description
Native Fibrinogen protein (Active)
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab92791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
Extinction coefficient: 1.51
Host species: Mouse
MW: 340.00 kDa
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.40
Constituent: 0.59% Sodium citrateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- FGA
- FGB
- FGG
see all -
Function
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
Post-translational
modificationsThe alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab92791 has been referenced in 2 publications.
- Jansen LE et al. Zwitterionic PEG-PC Hydrogels Modulate the Foreign Body Response in a Modulus-Dependent Manner. Biomacromolecules 19:2880-2888 (2018). PubMed: 29698603
- Waffarn EE et al. Infection-induced type I interferons activate CD11b on B-1 cells for subsequent lymph node accumulation. Nat Commun 6:8991 (2015). PubMed: 26612263
