Description

  • Product name

    Native Mouse Fibronectin protein
    See all Fibronectin proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    ab92784 is > 98% pure by SDS-PAGE. Purified from mouse plasma
  • Expression system

    Native
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Mouse
    • Predicted molecular weight

      220 kDa
    • Amino acids

      33 to 2477

Specifications

Our Abpromise guarantee covers the use of ab92784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Thaw the fibronectin by placing the vial in a 37°C water bath and leaving it undisturbed until completely thawed. Do not disturb the vial at any time during the thawing process. If the vial is disturbed or removed prior to complete thawing, the fibronectin will form a gel and be unusable. Mix very gently with pipette after thawing. Vortexing, excessive agitation, repeated freezing and thawing of fibronectin are not recommended.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituents: 1.58% Tris HCl, 0.87% Sodium chloride

General Info

  • Alternative names

    • CIG
    • Cold insoluble globulin
    • Cold-insoluble globulin
    • DKFZp686F10164
    • DKFZp686H0342
    • DKFZp686I1370
    • DKFZp686O13149
    • ED B
    • Fibronectin 1
    • FINC
    • FINC_HUMAN
    • FN
    • FN1
    • FNZ
    • GFND
    • GFND2
    • LETS
    • Migration stimulating factor
    • MSF
    • Ugl-Y3
    see all
  • Function

    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • Tissue specificity

    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • Involvement in disease

    Glomerulopathy with fibronectin deposits 2
  • Sequence similarities

    Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • Developmental stage

    Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • Post-translational
    modifications

    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt

Images

  • 10% SDS-PAGE
    Lane 1: Fibronectin (5 ug) Reduced
    Lane 2: Fibronectin (5 ug) Non-reduced
    Lane 3: Prestained Standard

References

This product has been referenced in:

  • Shen Y  et al. Recombinant Decorin Fusion Protein Attenuates Murine Abdominal Aortic Aneurysm Formation and Rupture. Sci Rep 7:15857 (2017). Read more (PubMed: 29158532) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

I can confirm that Mouse Fibronectin full length protein (ab92784) was prepared from fresh mouse plasma.



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Answer

Thank you for your enquiry.

Here is a link to our tissue culture guidelines:
https://www.abcam.com/index.html?pageconfig=resource&rid=11453

I hope this is helpful. Please contact us again if you have any further questions.

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Answer

For long term storage, aliquot and flash freeze on dry ice. Store at -70 C.

I hope this is helpful. Please contact me again if you have any further questions.

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