Description

  • Product name

    Native Mouse Vitronectin/S-Protein
    See all Vitronectin/S-Protein proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    ab92727 was prepared from fresh Mouse plasma using urea as a denaturant.
  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Mouse

Specifications

Our Abpromise guarantee covers the use of ab92727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituents: 0.82% Sodium phosphate, 0.58% Sodium chloride

General Info

  • Alternative names

    • Complement S Protein
    • Epibolin
    • S Protein
    • S-protein
    • Serum Spreading Factor
    • Serum-spreading factor
    • Somatomedin B
    • Somatomedin-B
    • V75
    • Vitronectin
    • Vitronectin V10 subunit
    • Vitronectin V65 subunit
    • VN
    • VNT
    • VTN
    • VTNC_HUMAN
    see all
  • Function

    Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • Tissue specificity

    Plasma.
  • Sequence similarities

    Contains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.
  • Domain

    The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • Post-translational
    modifications

    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization

    Secreted, extracellular space.
  • Information by UniProt

Images

  • 10% SDS-PAGE showing ab92727
    Lane 1 ab92727 Non-reduced (3µg)
    Lane 2 ab92727 Reduced (3µg)
    Lane 3 Molecular weight markers

References

This product has been referenced in:

  • Bae HB  et al. Vitronectin inhibits efferocytosis through interactions with apoptotic cells as well as with macrophages. J Immunol 190:2273-81 (2013). Functional Studies . Read more (PubMed: 23345331) »
  • Bae HB  et al. Vitronectin inhibits neutrophil apoptosis through activation of integrin-associated signaling pathways. Am J Respir Cell Mol Biol 46:790-6 (2012). Functional Studies ; Mouse . Read more (PubMed: 22281987) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Product ab92727 corresponds to a native mouse multimeric vitronectin, with a molecular weight of 75 kDa (monomer).

Read More
Application
SDS-PAGE
The overall purpose of this experiment is to determine the cleavage fragments of mouse vitronectin when incubated with mouse Granzyme B.

From left to right:

vitronectin vitronectin vitronectin + GrB vitronectin + GrB

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Verified customer

Submitted Oct 10 2014

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