Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Product nameNative pig Calpain 1 protein
See all Calpain 1 proteins and peptides
Greater than 1000 units per mg protein prior to freezing.
One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 minute at 25 °C.
Note: 1 caseinolytic unit=9 uorogenic units.
Purity> 95 % SDS-PAGE.
Prepared from porcine erythrocytes obtained from a USDA -certified collection center.
Protein lengthFull length protein
Predicted molecular weight110 kDa
Additional sequence informationPrepared from porcine erythrocytes.
Our Abpromise guarantee covers the use of ab198675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.039% Beta mercaptoethanol, 0.029% EDTA, 0.038% EGTA, 30% Glycerol, 0.21% Imidazole hydrochloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Ca2 activated neutral protease
- Calcium activated neutral proteinase
- Calcium activated neutral proteinase small subunit
FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Sequence similaritiesBelongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains.
Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
- Information by UniProt
4-12% Bis-Tris NuPAGE gel.
Lane 1. Calpain 1 - 5 μg (reduced / heated)
Lane 2. Calpain 1 - 10 μg (reduced / heated)
Lane 3. Calpain 1 - 20 μg (reduced / heated
Lane 4. Competitor’s - 10 μg (reduced / heated)
Lane 5. BRMW Standard
Lane 6. Calpain 1 - 5 μg (non-reduced / no heat)
Lane 7. Calpain 1 - 10 μg (non-reduced / no heat)
Lane 8. Calpain 1 - 10 μg (non-reduced / no heat)
Lane 9. Competitor’s - 10 μg (non-reduced / no heat)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198675 has not yet been referenced specifically in any publications.