Overview

Description

  • Nature

    Native
  • Source

    Native
  • Amino Acid Sequence
    • Accession
    • Species

      Pig
    • Molecular weight

      88 kDa
    • Amino acids

      1 to 766

Specifications

Our Abpromise guarantee covers the use of ab134449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific activity of ab134449 was >40 U /mg (H-Gly-Pro-amino-4-methylcoumarin or GlyL-Pro-p-nitroanilide substrate) enzyme dilution >1:100 / 5-15 µl for enzyme activity assay (100 µl) in 0.1 M Tris-HCl pH 8.5, incubation time 10 min at 37°C.
  • Applications

    Functional Studies

    Inhibition Assay

    SDS-PAGE

  • Purity

    > 90 % SDS-PAGE.

  • Form

    Liquid
  • Additional notes

    ab134449 can be kept at -20°C for several weeks and at 4°C for 1 week without significant loss of activity.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Preservative: 0.05% Sodium azide
    Constituents: 0.05% Calcium chloride, 0.0001% Zinc chloride, 0.32% Tris HCl

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CD26 antigen
    • ADA-binding protein
    • ADABP
    • ADCP 2
    • ADCP-2
    • ADCP2
    • Adenosine deaminase complexing protein 2
    • CD 26
    • CD26
    • CD26 antigen 3
    • Dipeptidyl peptidase 4
    • Dipeptidyl peptidase 4 soluble form
    • Dipeptidyl peptidase IV
    • Dipeptidyl peptidase IV membrane form
    • Dipeptidyl peptidase IV soluble form
    • Dipeptidyl peptidase, intestinal
    • Dipeptidylpeptidase 4
    • Dipeptidylpeptidase IV
    • Dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
    • DPP 4
    • DPP IV
    • DPP IV estoenzyme
    • DPP4
    • DPP4_HUMAN
    • DPPIV
    • Intestinal dipeptidyl peptidase
    • T cell activation antigen CD26
    • T-cell activation antigen CD26
    • TP 103
    • TP103
    see all
  • Function

    Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
  • Tissue specificity

    Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
  • Sequence similarities

    Belongs to the peptidase S9B family. DPPIV subfamily.
  • Domain

    The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity.
  • Post-translational
    modifications

    The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
    N- and O-Glycosylated.
    Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
  • Cellular localization

    Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab134449.

References

ab134449 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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