Native Protein A (HRP) (ab7456)

Submit a review Q&A (2)References (15)

Key features and details

  • Expression system: Native
  • Suitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy

Description

Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab7456 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      Dot blot

      ELISA

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

      Immunohistochemistry (Frozen sections)

      Immunomicroscopy

    • Form

      Liquid

    • Additional notes

      Molecular Weight (protein A): 42,000 daltons Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C.

      Preservative: 0.01% Gentamicin sulphate
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1.5% BSA

    General Info

    • Alternative names

      • gG-binding protein A
      • Immunoglobulin G binding protein A
      • SPA
      • Staphylococcal protein A

    • Relevance

      Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

    • Cellular localization

      Secreted; cell wall.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (15)

    Publishing research using ab7456? Please let us know so that we can cite the reference in this datasheet.

    ab7456 has been referenced in 15 publications.

    • Kuninaga N  et al. Serological and histological evaluation of species-specific immunocontraceptive vaccine antigens based on zona pellucida 3 in the small Indian mongoose (Herpestes auropunctatus). J Vet Med Sci 81:328-337 (2019). PubMed: 30587673
    • Mahajan D  et al. Dopey1-Mon2 complex binds to dual-lipids and recruits kinesin-1 for membrane trafficking. Nat Commun 10:3218 (2019). PubMed: 31324769
    • Liu J  et al. Topoisomerase inhibitors promote cancer cell motility via ROS-mediated activation of JAK2-STAT1-CXCL1 pathway. J Exp Clin Cancer Res 38:370 (2019). PubMed: 31438997
    • Shi M  et al. Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5. Nat Commun 9:4987 (2018). PubMed: 30478271
    • Pavlaki I  et al. The long non-coding RNA Paupar promotes KAP1-dependent chromatin changes and regulates olfactory bulb neurogenesis. EMBO J 37:N/A (2018). PubMed: 29661885
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Inquiry: Dear Sir/Madam We have purchased abcam Protein A HRP(ab 7456) My query is regarding the dilution of Protein A HRP(ab 7456) to be used in western blotting for development with DAB as substrate
    Thanking you Sincerely

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    Answer

    Thank you for your enquiry.

    The best dilution of ab7456 for your application will depend on thethe individual experiment which will require some individual optimization. However, I can suggest dilutions of from 1:1000 and 1:5000 are usually good starting points for optimization .

    I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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    Question

    band of interest is at about 27 kDda where light chain of endogenous IgG (from tissue samples) appears
    send reference for using protein A-HRP or protein G-HRP to not detect the heavy and light chain of endogenous IgG in WB

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    Answer

    Thank you for contacting us.

    Both protein A-HRP (e.g. ab7456) or protein G-HRP (e.g. ab7460) can be used to not detect the heavy and light chain of endogenous IgG in WB.


    The links to the datasheets for protein A (HRP) and protein G (HRP) - which would both work well with a rabbit primary antibody are here:

    https://www.abcam.com/Protein-A-protein-HRP-ab7456.html
    https://www.abcam.com/Protein-G-protein-HRP-ab7460.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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