Native Protein A (HRP) (ab7456)
Key features and details
- Expression system: Native
- Suitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy
Description
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Product name
Native Protein A (HRP)
See all Protein A proteins and peptides -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
Conjugation
HRP
Associated products
Specifications
Our Abpromise guarantee covers the use of ab7456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Dot blot
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunomicroscopy
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Form
Liquid -
Additional notes
Molecular Weight (protein A): 42,000 daltons Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14) -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1.5% BSA
General Info
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Alternative names
- gG-binding protein A
- Immunoglobulin G binding protein A
- SPA
- Staphylococcal protein A
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Relevance
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
Cellular localization
Secreted; cell wall.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (15)
ab7456 has been referenced in 15 publications.
- Kuninaga N et al. Serological and histological evaluation of species-specific immunocontraceptive vaccine antigens based on zona pellucida 3 in the small Indian mongoose (Herpestes auropunctatus). J Vet Med Sci 81:328-337 (2019). PubMed: 30587673
- Mahajan D et al. Dopey1-Mon2 complex binds to dual-lipids and recruits kinesin-1 for membrane trafficking. Nat Commun 10:3218 (2019). PubMed: 31324769
- Liu J et al. Topoisomerase inhibitors promote cancer cell motility via ROS-mediated activation of JAK2-STAT1-CXCL1 pathway. J Exp Clin Cancer Res 38:370 (2019). PubMed: 31438997
- Shi M et al. Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5. Nat Commun 9:4987 (2018). PubMed: 30478271
- Pavlaki I et al. The long non-coding RNA Paupar promotes KAP1-dependent chromatin changes and regulates olfactory bulb neurogenesis. EMBO J 37:N/A (2018). PubMed: 29661885