Native Protein A (HRP) (ab7456)

Submit a review Q&A (2)References (10)

Description

Specifications

Our Abpromise guarantee covers the use of ab7456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Dot blot

    ELISA

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemistry (Frozen sections)

    Immunomicroscopy

  • Form

    Liquid

  • Additional notes

    Molecular Weight (protein A): 42,000 daltons Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1.5% BSA

General Info

  • Alternative names

    • gG-binding protein A
    • Immunoglobulin G binding protein A
    • SPA
    • Staphylococcal protein A

  • Relevance

    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

  • Cellular localization

    Secreted; cell wall.

References

This product has been referenced in:

  • Kuninaga N  et al. Serological and histological evaluation of species-specific immunocontraceptive vaccine antigens based on zona pellucida 3 in the small Indian mongoose (Herpestes auropunctatus). J Vet Med Sci 81:328-337 (2019). Read more (PubMed: 30587673) »
  • Mahajan D  et al. Dopey1-Mon2 complex binds to dual-lipids and recruits kinesin-1 for membrane trafficking. Nat Commun 10:3218 (2019). Read more (PubMed: 31324769) »
See all 10 Publications for this product

Publishing research using ab7456? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Inquiry: Dear Sir/Madam We have purchased abcam Protein A HRP(ab 7456) My query is regarding the dilution of Protein A HRP(ab 7456) to be used in western blotting for development with DAB as substrate
Thanking you Sincerely

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Answer

Thank you for your enquiry.

The best dilution of ab7456 for your application will depend on thethe individual experiment which will require some individual optimization. However, I can suggest dilutions of from 1:1000 and 1:5000 are usually good starting points for optimization .

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

band of interest is at about 27 kDda where light chain of endogenous IgG (from tissue samples) appears
send reference for using protein A-HRP or protein G-HRP to not detect the heavy and light chain of endogenous IgG in WB

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Answer

Thank you for contacting us.

Both protein A-HRP (e.g. ab7456) or protein G-HRP (e.g. ab7460) can be used to not detect the heavy and light chain of endogenous IgG in WB.


The links to the datasheets for protein A (HRP) and protein G (HRP) - which would both work well with a rabbit primary antibody are here:

https://www.abcam.com/Protein-A-protein-HRP-ab7456.html
https://www.abcam.com/Protein-G-protein-HRP-ab7460.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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