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band of interest is at about 27 kDda where light chain of endogenous IgG (from tissue samples) appears
send reference for using protein A-HRP or protein G-HRP to not detect the heavy and light chain of endogenous IgG in WB
Asked on Mar 08 2012
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Both protein A-HRP (e.g. ab7456) or protein G-HRP (e.g. ab7460) can be used to not detect the heavy and light chain of endogenous IgG in WB.
The links to the datasheets for protein A (HRP) and protein G (HRP) - which would both work well with a rabbit primary antibody are here:
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Answered on Mar 08 2012