Our Abpromise guarantee covers the use of ab7458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications


  • Form

  • Additional notes

    Molecular Weight: 33,000 daltons Approximately 5 mg of human IgG can be bound by 1 mg of Protein G. Optimal binding of Protein G to antibodies occurs at pH 5-6, with elution at pH 2.5-3. This product was expressed recombinantly in E. coli (non-native).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.


  • Reconstitution
    Reconstitute with 1ml of distilled water to a concentration of 1mg/ml

General Info

  • Alternative names

    • IgG binding protein G
    • IgG-binding protein G
    • Immunoglobulin G binding protein G [Precursor]
    • spg
    • spg immunoglobulin G-binding protein
    • SPG2_STRSG
    see all
  • Relevance

    Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
  • Cellular localization

    Cell Wall and Secreted


ab7458 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


J'aimerais vous informer que mes collègues responsables de l'intégration des produits travaillent toujours à la recherche d'un collaborateur qui pourrait nous fournir :
- une protéine G (full length sans tag) en complément de la référence ab7458 (protéine G, full length, His tag),
- une protéine A (full length avec His-tag) en complément de la référence ab117657 (protéine A, full length, sans tag)

Je vous contacterai de nouveau avec les informations que mes collègues m'auront fournies.

Merci pour votre patience et bon week-end.

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Thank you for your enquiry. The immunogen is Protein A from S aureus. This product is an antibody against Protein A from S. aureus. I am sorry but I do not have a reference where this product was used. If a reference becomes available we will certainly publish it on the online datasheet. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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