Key features and details
- Expression system: Native
- Suitable for: Conjugation
Product nameNative Protein G
See all Protein G proteins and peptides
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab7458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesMolecular Weight: 33,000 daltons Approximately 5 mg of human IgG can be bound by 1 mg of Protein G. Optimal binding of Protein G to antibodies occurs at pH 5-6, with elution at pH 2.5-3. This product was expressed recombinantly in E. coli (non-native).
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
ReconstitutionReconstitute with 1ml of distilled water to a concentration of 1mg/ml
- IgG binding protein G
- IgG-binding protein G
- Immunoglobulin G binding protein G [Precursor]
RelevanceProtein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
Cellular localizationCell Wall and Secreted
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab7458 has not yet been referenced specifically in any publications.