Key features and details
- Expression system: Native
- Purity: > 98% Protein G purified
- Suitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr
Product nameNative Protein G (Alkaline Phosphatase)
See all Protein G proteins and peptides
Purity> 98 % Protein G purified.
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab7461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Additional notesMolecular Weight (protein G): 33,000 daltons Extinction Coefficient: 1% at 280 nm is 9.5(E0.1% of 9.5)
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
- IgG binding protein G
- IgG-binding protein G
- Immunoglobulin G binding protein G [Precursor]
RelevanceProtein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
Cellular localizationCell Wall and Secreted
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab7461 has been referenced in 1 publication.
- Zheng W & Yu A EZH2-mediated suppression of lncRNA-LET promotes cell apoptosis and inhibits the proliferation of post-burn skin fibroblasts. Int J Mol Med 41:1949-1957 (2018). PubMed: 29393360