• Product name

    Native Protein G (Alkaline Phosphatase)
    See all Protein G proteins and peptides
  • Expression system

  • Protein length

    Full length protein
  • Animal free

  • Nature

  • Conjugation

    Alkaline Phosphatase


Our Abpromise guarantee covers the use of ab7461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Dot blot


    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemistry (Frozen sections)

  • Form

  • Additional notes

    Molecular Weight (protein G): 33,000 daltons Extinction Coefficient: 1% at 280 nm is 9.5(E0.1% of 9.5)
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride

General Info

  • Alternative names

    • IgG binding protein G
    • IgG-binding protein G
    • Immunoglobulin G binding protein G [Precursor]
    • spg
    • spg immunoglobulin G-binding protein
    • SPG2_STRSG
    see all
  • Relevance

    Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
  • Cellular localization

    Cell Wall and Secreted


This product has been referenced in:

  • Zheng W & Yu A EZH2-mediated suppression of lncRNA-LET promotes cell apoptosis and inhibits the proliferation of post-burn skin fibroblasts. Int J Mol Med 41:1949-1957 (2018). Read more (PubMed: 29393360) »
See 1 Publication for this product

Customer reviews and Q&As


Thank you for your enquiry. Here are the answers to your questions. 1) What is the format of this protein G (e.g Fc region only, albumin domain only) Answer: Recombinant Protein G containing IgG binding domain 2) Do you have any reference on the use of this product? Answer: No references available 3) What is the affinity constant, dissociative rate constant ? Do you have any data for this? Answer: The affinity constant is not determined 4) How much is the relative specific activity of AP? Please also explain about the activity and use of AP for conjugation. Answer: The specific activity of the AP is not determined after conjugation. Before conjugation, the Alkaline phosphatase has an activity between 7000 and 8000 units per mg. Unit definition is amount of enzyme causing hydrolysis of one micromole of p-nitrophenylphosphate per minute at 25 degrees C pH 9.6. 5) What is the ratio of Protein G to AP? Answer: 3-5 moles protein G per mole of Alkaline Phosphatase

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