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Can you please provide information regarding the following questions?
Asked on May 18 2011
Thank you for your enquiry. Here are the answers to your questions. 1) What is the format of this protein G (e.g Fc region only, albumin domain only) Answer: Recombinant Protein G containing IgG binding domain 2) Do you have any reference on the use of this product? Answer: No references available 3) What is the affinity constant, dissociative rate constant ? Do you have any data for this? Answer: The affinity constant is not determined 4) How much is the relative specific activity of AP? Please also explain about the activity and use of AP for conjugation. Answer: The specific activity of the AP is not determined after conjugation. Before conjugation, the Alkaline phosphatase has an activity between 7000 and 8000 units per mg. Unit definition is amount of enzyme causing hydrolysis of one micromole of p-nitrophenylphosphate per minute at 25 degrees C pH 9.6. 5) What is the ratio of Protein G to AP? Answer: 3-5 moles protein G per mole of Alkaline Phosphatase
Answered on May 18 2011