Description

  • Product name

    Native Rabbit Actin protein
    See all Actin proteins and peptides
  • Purity

    > 98 % SDS-PAGE.
    Purity:Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Rabbit
    • Additional sequence information

      Purified from rabbit striated muscle

Specifications

Our Abpromise guarantee covers the use of ab73630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

    ab73630 can be used in applications as a: Protein standard in 1D and 2D SDS gel electrophoresis Immunoassays Immunization

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.00222% Calcium chloride, 0.5% SDS, 0.0154% DTT, 0.158% Tris HCl, 0.01014% ATP

  • Reconstitution
    Reconstitute in sterile 18MOhm/cm water to not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names

    • a actin
    • a-actin
    • ACTA
    • ACTA1
    • Actin
    • Actin alpha skeletal muscle
    • actin, alpha 1, skeletal muscle
    • actin, alpha 1, skeletal muscle 1
    • Actin, alpha skeletal muscle
    • actina
    • actine
    • ACTS_HUMAN
    • aktin
    • Alpha Actin 1
    • alpha skeletal muscle
    • Alpha skeletal muscle Actin
    • alpha-actin
    • Alpha-actin-1
    • ASMA
    • CFTD
    • CFTD1
    • CFTDM
    • MPFD
    • NEM1
    • NEM2
    • NEM3
    • nemaline myopathy type 3
    see all
  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

Images

  • Anti-Actin antibody - Loading Control (ab1801) at 1/1000 dilution + Native Rabbit Actin protein (ab73630) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 4 minutes

References

ab73630 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you so much for your colleague's phone call. I am sending the discount code for the actin protein ab73630. DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab73630 in WB. This code will give you 1 free PROTEIN before the expiration date. To redeem this offer, please submit an Abreview for WBand include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you for your inquiry. This actin protein (ab73630) was purified from rabbit striated muscle and the purification method used results in a highly purified protein. Unfortunately, the protein of the muscle consists in the alpha-skeletal muscle isoform and is therefore not suitable as a positive control for a beta actin antibody. I hope this information is nevertheless helpful to you. Please do not hesitate to contact us again with any other questions.

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