Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
Product nameNative rat Antithrombin III/ATIII protein
See all Antithrombin III/ATIII proteins and peptides
Biological activityab93317 is 98% active by Thrombin titration.
Purity> 95 % SDS-PAGE.
ab93317 is purified using several chromatographic steps and is > 98% pure by SDS-PAGE.
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab93317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesSolubility: > 5 mg/mL
Spectrophotometric Data: Ultraviolet: Absorbance (280nm) = 0.52 epsilon0.1% = 0.65 Concentration = 0.8 mg/mL
Previously labelled as Antithrombin III.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.82% Sodium phosphate, 0.0292% EDTA, 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Antithrombin III
FunctionMost important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
Tissue specificityFound in plasma.
Involvement in diseaseDefects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
Sequence similaritiesBelongs to the serpin family.
modificationsPhosphorylation sites are present in the extracelllular medium.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab93317 has not yet been referenced specifically in any publications.