Native Rat Thrombin protein (ab185262)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
Description
-
Product name
Native Rat Thrombin protein
See all Thrombin proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Native -
-
Species
Rat -
Sequence
TFGLGEADCGLRPLFEKKSLTDKTEKELLDSYIDGRIVEGWDAEKGIAPW QVMLFRKSPQELLCGASLISDRWVLTAAHCILYPPWDKNFTENDLLVRIG KHSRTRYERNVEKISMLEKIYIHPRYNWRENLDRDIALLKLKKPVPFSDY IHPVCLPDKQTVTSLLQAGYKGRVTGWGNLRETWTTNINEIQPSVLQVVN LPIVERPVCKASTRIRITDNMFCAGFKVNDTKRGDACEGDSGGPFVMKSP YNHRWYQMGIVSWGEGCDRNGKYGFYTHVFRLKRWMQKVIDQHR -
Predicted molecular weight
37 kDa -
Amino acids
324 to 617 -
Additional sequence information
Prepared from purified rat prothrombin by activation with Russells Viper Venom. This venom is removed after activation.
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab185262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Form
Liquid -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Constituents: 0.0132% Sodium citrate, 1.17% Sodium chloride, 0.1% Polyethylene glycol
General Info
-
Alternative names
- Coagulation factor II
- Coagulation factor II thrombin
- EC 3.4.21.5
see all -
Function
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Factor II deficiency
Ischemic stroke
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2 -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). -
Cellular localization
Secreted, extracellular space. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab185262 has not yet been referenced specifically in any publications.