Description

  • Product name

    Native Staphylococcus aureus Protein A (FITC)
    See all Protein A proteins and peptides
  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Staphylococcus aureus
    • Predicted molecular weight

      56 kDa
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Specifications

Our Abpromise guarantee covers the use of ab7455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Flow Cytometry

    Immunomicroscopy

  • Form

    Liquid
  • Additional notes

    Molecular Weight (protein A): 42,000 daltons Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, BSA

General Info

  • Alternative names

    • gG-binding protein A
    • Immunoglobulin G binding protein A
    • SPA
    • Staphylococcal protein A
  • Relevance

    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localization

    Secreted; cell wall.

References

This product has been referenced in:

  • He B  et al. Group A Rotaviruses in Chinese Bats: Genetic Composition, Serology, and Evidence for Bat-to-Human Transmission and Reassortment. J Virol 91:N/A (2017). IF . Read more (PubMed: 28381569) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you very much for having patience.

My colleague has tried to source these products form our collaborator supplier however due to non availability of these product we are unable to add these in catalogue.

You can however use the following kits to conjugate the protein yourselves. Please check the following products

ab102851, ab102849, ab102850, ab102848

ab102912, ab102870

Please click the below given link for full list;

https://www.abcam.com/index.html?pageconfig=resource&rid=13148

We unfortunately do not have recombinant anti human IgG1 antibody available.

I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

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Answer

Thank you for contacting us.

I can confirm that I have forwarded your inquiry to my colleague, who is protein product manager at Abcam. She is now looking into the requested product line and will be contacting me with updates soon.

I will get back to you, as soon as I get a reply from her.

Thank you very much for having patience!

Read More

Answer

Thank you for contacting us. To my knowledge we do not have a prepared certificate of origin document for this protein. However, this product was derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. I hope this helps, please let me know if you need any other information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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