Overview

Description

  • Nature

    Native
  • Source

    Native
  • Amino Acid Sequence
  • Conjugation

    PerCP. Ex: 482nm, Em: 675nm

Specifications

Our Abpromise guarantee covers the use of ab134347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Flow Cytometry

    Western blot

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Store In the Dark.

    pH: 7.40
    Preservative: 0.013% Sodium azide
    Constituents: Phosphate Buffer, 1.71% Sucrose, 0.88% Sodium chloride, 0.1% BSA

  • Reconstitution
    Prior to use reconstitute to 1 ml with distilled deionized water, vortex and allow it to sit on ice for 20 minutes.

General Info

  • Alternative names

    • SA V1
    • SA V2
    • strepavidin
    • Streptavidin V1
    • Streptavidin V2
    see all
  • Relevance

    Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • Cellular localization

    Cytoplasmic

References

ab134347 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Flow Cytometry
Normal human dermal fibroblasts stained with CD140a-biotin (1:200) for 20 min at RT in 2% HS, 0.5 mM EDTA and washed 2x. Cells then washed with Streptavidin-PerCP (1:50) for 20 min at RT in 2% HS, 0.5 mM EDTA and washed 2x and analyzed.

Abcam user community

Verified customer

Submitted Nov 26 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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