Product nameAnti-Natriuretic Peptide Receptor A / GC-A antibody
See all Natriuretic Peptide Receptor A / GC-A primary antibodies
DescriptionRabbit polyclonal to Natriuretic Peptide Receptor A / GC-A
Tested applicationsSuitable for: WB, IHC-P, ICC, ICC/IF, Flow Cyt, IHC-Frmore details
Species reactivityReacts with: Rat, Human
Previously labelled as Natriuretic Peptide Receptor A.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab14356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Predicted molecular weight: 118 kDa.Can be blocked with Human Natriuretic Peptide Receptor A / GC-A peptide (ab28437).|
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionReceptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
Sequence similaritiesBelongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 1 guanylate cyclase domain.
Contains 1 protein kinase domain.
modificationsPhosphorylation of the protein kinase-like domain is required for full activation by ANP.
- Information by UniProt
- ANP-A antibody
- ANPa antibody
- ANPR-A antibody
ICC/IF image of ab14356 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14356, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- You H & Laychock SG Long-term treatment with atrial natriuretic peptide inhibits ATP production and insulin secretion in rat pancreatic islets. Am J Physiol Endocrinol Metab 300:E435-44 (2011). WB ; Rat . Read more (PubMed: 20959527) »
- Matera MG et al. Epithelium integrity is crucial for the relaxant activity of brain natriuretic peptide in human isolated bronchi. Br J Pharmacol 163:1740-54 (2011). Read more (PubMed: 21410689) »