Product nameAnti-Natriuretic Peptide Receptor A / GC-A antibody
See all Natriuretic Peptide Receptor A / GC-A primary antibodies
DescriptionRabbit polyclonal to Natriuretic Peptide Receptor A / GC-A
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- This antibody gave a positive signal in Kidney (Human) Tissue Lysate - adult normal tissue
Previously labelled as Natriuretic Peptide Receptor A.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab40817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 119 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionReceptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
Sequence similaritiesBelongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 1 guanylate cyclase domain.
Contains 1 protein kinase domain.
modificationsPhosphorylation of the protein kinase-like domain is required for full activation by ANP.
- Information by UniProt
- ANP-A antibody
- ANPa antibody
- ANPR-A antibody
Anti-Natriuretic Peptide Receptor A / GC-A antibody (ab40817) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 119 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab40817 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40817, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab40817 has not yet been referenced specifically in any publications.