Anti-NPR-A antibody (ab14356)
Key features and details
- Rabbit polyclonal to NPR-A
- Suitable for: ICC, IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NPR-A antibody -
Description
Rabbit polyclonal to NPR-A -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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ICC/IF |
1/300.
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Flow Cyt | (1) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB | (1) |
Use at an assay dependent concentration.
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Notes |
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ICC
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/300. |
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
Target
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Function
Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand. -
Sequence similarities
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 1 guanylate cyclase domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation of the protein kinase-like domain is required for full activation by ANP. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4881 Human
- Entrez Gene: 24603 Rat
- Omim: 108960 Human
- SwissProt: P16066 Human
- SwissProt: P18910 Rat
- Unigene: 490330 Human
- Unigene: 10463 Rat
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Alternative names
- ANP-A antibody
- ANPa antibody
- ANPR-A antibody
see all
Images
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ICC/IF image of ab14356 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14356, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (13)
ab14356 has been referenced in 13 publications.
- Chen Y et al. Retinal degeneration protein 3 controls membrane guanylate cyclase activities in brain tissue. Front Mol Neurosci 15:1076430 (2022). PubMed: 36618828
- Zhu XL et al. High salt-induced weakness of anti-oxidative function of natriuretic peptide receptor-C and podocyte damage in the kidneys of Dahl rats. Chin Med J (Engl) 133:1182-1191 (2020). PubMed: 32433050
- Meng J et al. New mechanism underlying IL-31-induced atopic dermatitis. J Allergy Clin Immunol 141:1677-1689.e8 (2018). PubMed: 29427643
- Moubarak M et al. Effects of atrial natriuretic peptide on rat ventricular fibroblasts during differentiation into myofibroblasts. Physiol Res 64:495-503 (2015). PubMed: 25470517
- You H & Laychock SG Long-term treatment with atrial natriuretic peptide inhibits ATP production and insulin secretion in rat pancreatic islets. Am J Physiol Endocrinol Metab 300:E435-44 (2011). WB ; Rat . PubMed: 20959527